The investigation of gene flows in artificial pollination between GM rice and its wild relatives by RAPD analysis = RAPD PCR에 의한 GM벼의 야생 근연종 벼로의 유전자 전이 분석법

Abstract

In recent years, there has been increasing concerns in gene flow fromGMcrops towild or weedy relatives as a potential risk in the commercialization of GMcrops. To access the possibility of the environmental impacts by GMrice, small-scale experiments of gene transfer were carried out. Herbicide and drought stress resistant GMrice and non-GMrice Nakdongbyeo, wild rice Oryza nivara, andweedy rice Sharebyeowere used for artificial pollination experiments and bar genewas used as a tractablemarker after pollination. The harvested putative hybrid seeds after artificial pollination were germinated and true hybrid plants were selected by basta treatment. The hybrid plants were verified again by PCR amplification of bar and trehalose-6-phosphate phosphatase (TPP) genes and RAPD PCR analysis.