Expressed sequence tag analysis of the diapausing queen of the bumblebee Bombus ignitus

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Title
Expressed sequence tag analysis of the diapausing queen of the bumblebee Bombus ignitus
Author(s)
Y J Kim; J S Hwang; H J Yoon; E Y Yun; S B Lee; M Y Ahn; Nam-Soon Kim; I Kim; J P Jeon; S J Hwang
Bibliographic Citation
Entomological Research, vol. 36, no. 4, pp. 191-195
Publication Year
2006
Abstract
We constructed a full-length cDNA library from diapausing queens of the bumblebee Bombus ignitus. A total of 480 randomly selected clones was sequenced by singlerun5′-end sequencing. of these, there were 437 high quality clones, 23 poor quality clones and 20 read-fail clones. Each high quality clone sequence was searched against a public protein database. The most frequently found matching genes were ribosomal proteins (12.5%), p10 (3.58%), cytochrome P450 monooxygenase (3.13%) and sensory appendage protein (2.9%). Sequence similarity analysis between bumblebees and other insect species showed that 72 out of 437 (16.5%) bumblebee expressed sequence tags (EST) matched sequences of Apis mellifera , with matches to Drosophila melanogaster (6.6%), Caenorhabditis briggsae (6.2%), Lysiphlebus testaceipes (4.8%), Periplaneta americana (3.7%) and Anopheles gambiae (3.4%) following, suggesting that sequence similarity of bumblebee EST is closest to that of A. mellifera . Functional classification of EST based on Gene Ontology showed that most genes found by sequencing are associated with physiological processes in the bumblebee. The results of sequencing and analysis of our 437 cDNA demonstrated that high-throughput EST sequencing and data analysis are powerful means for identifying novel genes and for expression profiling. Our bumblebee EST collection could be a useful platform for further studies of gene expression in diapausing bumblebees.
Keyword
bombus ignitusdiapauseexpressed sequence tag (EST)
ISSN
1738-2297
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1748-5967.2006.00033.x
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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