Crystallization and initial crystallographic characterization of the Corynebacterium glutamicum nitrilotriacetate monooxygenase component A

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Title
Crystallization and initial crystallographic characterization of the Corynebacterium glutamicum nitrilotriacetate monooxygenase component A
Author(s)
K J Kim; SuJin Kim; S Lee; B S Kang; H S Lee; Tae Kwang Oh; Myung Hee Kim
Bibliographic Citation
Acta Crystallographica Section F-Structural Biology, vol. 62, no. 11, pp. 1141-1143
Publication Year
2006
Abstract
Safety and environmental concerns have recently dictated the proper disposal of nitrilotriacetate (NTA). Biodegradation of NTA is initiated by NTA monooxygenase, which is composed of two proteins: component A and component B. The NTA monooxygenase component A protein from Corynebacterium glutamicum was crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium sulfate as the precipitant. X-ray diffraction data were collected to a maximum resolution of 2.5 ? on a synchrotron beamline. The crystal belongs to the monoclinic space group C2, with unit-cell parameters a = 111.04, b = 98.51, c = 171.61 ?, β = 101.94°. The asymmetric unit consists of four molecules, corresponding to a packing density of 2.3 ?3 Da-1. The structure was solved by molecular replacement. Structure refinement is in progress.
Keyword
NitrilotriacetateNitrilotriacetate monooxygenase component A
ISSN
1744-3091
Publisher
Int Union Crystallography
DOI
http://dx.doi.org/10.1107/S1744309106042072
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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