Characterization of eight Rumex species by FISH (fluorescence in situ hybridization) and 5S rDNA spacer sequences

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Title
Characterization of eight Rumex species by FISH (fluorescence in situ hybridization) and 5S rDNA spacer sequences
Author(s)
Soo-Young Kim; H W Choi; D H Koo; W K Lee; Joongku Lee; J W Bang
Bibliographic Citation
Genes & Genomics, vol. 28, no. 3, pp. 243-251
Publication Year
2006
Abstract
Using both molecular and cytogenetic methods, we have examined and characterized eight Rumex species distributed throughout the Korean Peninsula. The somatic metaphase chromosomes in the Lapathum group were 2n=6x=60 in three of the species (R. crispus, R. japonicus and R. longifolius), 2n=5x=50 in one species (R. nipponicus), and 2n=4x=40 in two of the species (R. obtusifolius and R. maritinus). In the two dioecious groups, the chromosome numbers were 2n=6x=42 (female) and 43 (male) in R. acetocella, and 2n=2x=14 (female) and 15 (male) in R. acetosa. Using the FISH method, we physically mapped the 5S and 45S rDNA genes on the chromosomes of all the studied species. All of the species, except R. japonicus, possessed four 45S rDNA sites, whereas the 5S rDNA gene sites were variable, ranging between 2 and 8. Polymorphic 5S rDNA sites at the same ploidy level were found among species in the subgenus Lapathum group. Nucleotide sequence analysis revealed that the 5S rDNA spacer also varied between the species, ranging between 308 and 315 bp, except for each 120 bp coding region. FISH and 5S rDNA spacer sequencing were successfully applied to the identification and characterization of the eight Rumex species examined in this study.
Keyword
5S rDNA spacer sequenceFISHrDNAsRumex
ISSN
1976-9571
Publisher
Springer
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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