Development of bioreactor system for L-tyrosine synthesis using thermostable tyrosine phenol-lyase = 내열성 tyrosine phenol-lyase를 이용한 L-tyrosine 합성 생물반응시스템의 개발

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dc.contributor.authorDo Young Kim-
dc.contributor.authorEugene Rha-
dc.contributor.authorSu-Lim Choi-
dc.contributor.authorJae Jun Song-
dc.contributor.authorS P Hong-
dc.contributor.authorM H Sung-
dc.contributor.authorSeung Goo Lee-
dc.date.accessioned2017-04-19T09:05:35Z-
dc.date.available2017-04-19T09:05:35Z-
dc.date.issued2007-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7679-
dc.description.abstractAn efficient enzyme system for the synthesis of L-tyrosine was developed using a fed-batch reactor with continuous feeding of phenol, pyruvate, and ammonia. A thermo- and chemostable tyrosine phenol-lyase from Symbiobacterium toebii was employed as the biocatalyst in this work. The enzyme was produced using a constitutive expression system in Escherichia coli BL21, and prepared as a soluble extract by rapid clarification, involving treatment with 40% methanol in the presence of excess ammonium chloride. The stability of the enzyme was maintained for at least 18 h under the synthesis conditions, including 75 mM phenol at pH 8.5 and 40°C. The fed-batch system (working volume, 0.5 ?1) containing 1.0 kU of the enzyme preparation was continuously fed with two substrate preparations: one containing 2.2 M phenol and 2.4 M sodium pyruvate, and the other containing 0.4 mM pyridoxal-5-phosphate and 4 M ammonium chloride (pH 8.5). The system produced 130 g/l of L-tyrosine within 30 h, mostly as precipitated particles, upon continuous feeding of the substrates for 22 h. The maximum conversion yield of L-tyrosine was 94% on the basis of the supplied phenol.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleDevelopment of bioreactor system for L-tyrosine synthesis using thermostable tyrosine phenol-lyase = 내열성 tyrosine phenol-lyase를 이용한 L-tyrosine 합성 생물반응시스템의 개발-
dc.title.alternativeDevelopment of bioreactor system for L-tyrosine synthesis using thermostable tyrosine phenol-lyase-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number1-
dc.citation.endPage122-
dc.citation.startPage116-
dc.citation.volume17-
dc.contributor.affiliatedAuthorDo Young Kim-
dc.contributor.affiliatedAuthorEugene Rha-
dc.contributor.affiliatedAuthorSu-Lim Choi-
dc.contributor.affiliatedAuthorJae Jun Song-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.alternativeName김도영-
dc.contributor.alternativeName나유진-
dc.contributor.alternativeName최수림-
dc.contributor.alternativeName송재준-
dc.contributor.alternativeName홍승표-
dc.contributor.alternativeName성문희-
dc.contributor.alternativeName이승구-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 17, no. 1, pp. 116-122-
dc.subject.keywordbioconversion-
dc.subject.keywordfed-batch-
dc.subject.keywordL-tyrosine-
dc.subject.keywordsymbiobacterium toebii-
dc.subject.keywordtyrosine phenol-lyase-
dc.subject.localBio-conversion-
dc.subject.localbioconversion-
dc.subject.localBioconversion-
dc.subject.localfed-batch-
dc.subject.localFed-batch-
dc.subject.localL-tyrosine-
dc.subject.localsymbiobacterium toebii-
dc.subject.localSymbiobacterium toebii-
dc.subject.localTyrosine phenollyase-
dc.subject.localtyrosine phenol lyase-
dc.subject.localtyrosine phenol-lyase-
dc.subject.localTyrosine phenol-lyase-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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