ApoA-I mutants V156K and R173C promote anti-inflammatory function and antioxidant activities = 아포A-I 변이체 V156K와 R173C의 항염증기능과 항산화작용 조절

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dc.contributor.authorK H Cho-
dc.contributor.authorS H Park-
dc.contributor.authorJong Min Han-
dc.contributor.authorHyoung-Chin Kim-
dc.contributor.authorY K Choi-
dc.contributor.authorI Choi-
dc.date.accessioned2017-04-19T09:05:36Z-
dc.date.available2017-04-19T09:05:36Z-
dc.date.issued2006-
dc.identifier.issn00142972-
dc.identifier.uri10.1111/j.1365-2362.2006.01737.xko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7686-
dc.description.abstractBackground: Two mutants of apolipoprotein (apo) A-I, V156K and A158E, showed markedly different structural and functional properties in lipid-free and lipid-bound states in the authors' earlier report. The physiological activities of these mutants were compared with the wild-type (WT) and R173C mutant using in vitro and in vivo experiments. Materials and methods: A reconstituted high-density lipoprotein (rHDL) with palmitoyloleoyl phosphatidylcholine (POPC), combined with each of the apoA-I variants, was injected into the tail-veins of hypercholesterolaemic mice (C57BL6/J), which had been fed a high cholesterol and high fat (HCHF; 0.5% cholesterol, 15% lard, 0.1% sodium cholate) diet for 23 weeks, once at 0 h and then every 24 h, at a dosage of 30 mg apoA-I kg -1 of body-weight. Results: The V156K-rHDL and R173C-rHDL exhibited significantly stronger anti-oxidant activity against copper-mediated low-density lipoprotein (LDL) oxidation than did A158E in an apolipoprotein state. The mice injected with WT-rHDL or A158E-rHDL showed abrupt increases in total cholesterol concentrations (47% and 38%, respectively) as compared with the levels before injection, whereas the mice injected with V156K-rHDL and R173C-rHDL did not. Injection with V156K-rHDL improved serum lipids and anti-oxidative activities compared with the injection of WT-rHDL. Injection of WT-rHDL or A158E-rHDL increased serum interleukin-6 (IL-6) to 90-110 pg mL -1, whereas the injection of V156K-rHDL or R173C-rHDL increased serum IL-6 to 17-25 pg mL-1 only. Conclusion: The V156K-rHDL and R173C-rHDL displayed potent beneficial effects, including anti-oxidant and anti-inflammatory activity from both in vitro and in vivo evaluations, whereas the WT-rHDL and A158E-rHDL did not.-
dc.publisherWiley-
dc.titleApoA-I mutants V156K and R173C promote anti-inflammatory function and antioxidant activities = 아포A-I 변이체 V156K와 R173C의 항염증기능과 항산화작용 조절-
dc.title.alternativeApoA-I mutants V156K and R173C promote anti-inflammatory function and antioxidant activities-
dc.typeArticle-
dc.citation.titleEuropean Journal of Clinical Investigation-
dc.citation.number12-
dc.citation.endPage882-
dc.citation.startPage875-
dc.citation.volume36-
dc.contributor.affiliatedAuthorHyoung-Chin Kim-
dc.contributor.alternativeName조경현-
dc.contributor.alternativeName박순현-
dc.contributor.alternativeName한종민-
dc.contributor.alternativeName김형진-
dc.contributor.alternativeName최양규-
dc.contributor.alternativeName최인호-
dc.identifier.bibliographicCitationEuropean Journal of Clinical Investigation, vol. 36, no. 12, pp. 875-882-
dc.identifier.doi10.1111/j.1365-2362.2006.01737.x-
dc.subject.keywordApolipoprotein A-I-
dc.subject.keywordHDL-therapy-
dc.subject.keywordPoint mutant-
dc.subject.keywordReconstituted HDL-
dc.subject.localApolipoprotein A-I-
dc.subject.localHDL-therapy-
dc.subject.localPoint mutant-
dc.subject.localReconstituted HDL-
dc.description.journalClassY-
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