DC Field | Value | Language |
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dc.contributor.author | K H Cho | - |
dc.contributor.author | S H Park | - |
dc.contributor.author | Jong Min Han | - |
dc.contributor.author | Hyoung-Chin Kim | - |
dc.contributor.author | Y K Choi | - |
dc.contributor.author | I Choi | - |
dc.date.accessioned | 2017-04-19T09:05:36Z | - |
dc.date.available | 2017-04-19T09:05:36Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0014-2972 | - |
dc.identifier.uri | 10.1111/j.1365-2362.2006.01737.x | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7686 | - |
dc.description.abstract | Background: Two mutants of apolipoprotein (apo) A-I, V156K and A158E, showed markedly different structural and functional properties in lipid-free and lipid-bound states in the authors' earlier report. The physiological activities of these mutants were compared with the wild-type (WT) and R173C mutant using in vitro and in vivo experiments. Materials and methods: A reconstituted high-density lipoprotein (rHDL) with palmitoyloleoyl phosphatidylcholine (POPC), combined with each of the apoA-I variants, was injected into the tail-veins of hypercholesterolaemic mice (C57BL6/J), which had been fed a high cholesterol and high fat (HCHF; 0.5% cholesterol, 15% lard, 0.1% sodium cholate) diet for 23 weeks, once at 0 h and then every 24 h, at a dosage of 30 mg apoA-I kg -1 of body-weight. Results: The V156K-rHDL and R173C-rHDL exhibited significantly stronger anti-oxidant activity against copper-mediated low-density lipoprotein (LDL) oxidation than did A158E in an apolipoprotein state. The mice injected with WT-rHDL or A158E-rHDL showed abrupt increases in total cholesterol concentrations (47% and 38%, respectively) as compared with the levels before injection, whereas the mice injected with V156K-rHDL and R173C-rHDL did not. Injection with V156K-rHDL improved serum lipids and anti-oxidative activities compared with the injection of WT-rHDL. Injection of WT-rHDL or A158E-rHDL increased serum interleukin-6 (IL-6) to 90-110 pg mL -1, whereas the injection of V156K-rHDL or R173C-rHDL increased serum IL-6 to 17-25 pg mL-1 only. Conclusion: The V156K-rHDL and R173C-rHDL displayed potent beneficial effects, including anti-oxidant and anti-inflammatory activity from both in vitro and in vivo evaluations, whereas the WT-rHDL and A158E-rHDL did not. | - |
dc.publisher | Wiley | - |
dc.title | ApoA-I mutants V156K and R173C promote anti-inflammatory function and antioxidant activities = 아포A-I 변이체 V156K와 R173C의 항염증기능과 항산화작용 조절 | - |
dc.title.alternative | ApoA-I mutants V156K and R173C promote anti-inflammatory function and antioxidant activities | - |
dc.type | Article | - |
dc.citation.title | European Journal of Clinical Investigation | - |
dc.citation.number | 12 | - |
dc.citation.endPage | 882 | - |
dc.citation.startPage | 875 | - |
dc.citation.volume | 36 | - |
dc.contributor.affiliatedAuthor | Jong Min Han | - |
dc.contributor.affiliatedAuthor | Hyoung-Chin Kim | - |
dc.contributor.alternativeName | 조경현 | - |
dc.contributor.alternativeName | 박순현 | - |
dc.contributor.alternativeName | 한종민 | - |
dc.contributor.alternativeName | 김형진 | - |
dc.contributor.alternativeName | 최양규 | - |
dc.contributor.alternativeName | 최인호 | - |
dc.identifier.bibliographicCitation | European Journal of Clinical Investigation, vol. 36, no. 12, pp. 875-882 | - |
dc.identifier.doi | 10.1111/j.1365-2362.2006.01737.x | - |
dc.subject.keyword | Apolipoprotein A-I | - |
dc.subject.keyword | HDL-therapy | - |
dc.subject.keyword | Point mutant | - |
dc.subject.keyword | Reconstituted HDL | - |
dc.subject.local | Apolipoprotein A-I | - |
dc.subject.local | apolipoprotein A-I | - |
dc.subject.local | HDL-therapy | - |
dc.subject.local | Point mutant | - |
dc.subject.local | reconstituted HDL | - |
dc.subject.local | Reconstituted HDL | - |
dc.description.journalClass | Y | - |
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