Monoclonal anti-thrombopoietin antibodies generated by genetic immunization

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dc.contributor.authorN K Lim-
dc.contributor.authorJ H Kim-
dc.contributor.authorS Y Kim-
dc.contributor.authorH J Kang-
dc.contributor.authorK S Kim-
dc.contributor.authorS Lee-
dc.contributor.authorHyo Jeong Hong-
dc.contributor.authorK S Inn-
dc.date.accessioned2017-04-19T09:05:38Z-
dc.date.available2017-04-19T09:05:38Z-
dc.date.issued2006-
dc.identifier.issn0272457X-
dc.identifier.uri10.1089/hyb.2006.25.75ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7695-
dc.description.abstractThrombopoietin (TPO) is a megakaryocyte growth and differentiation factor that is currently being investigated as a therapeutic for cancer patients undergoing myelosuppressive chemotherapy. We generated monoclonal antibodies (MAbs) specific for human thrombopoietin (hTPO) by genetic immunization using an hTPO expression plasmid and an adjuvant plasmid that encodes mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). All genetically immunized mice exhibited a high humoral immune response. Splenocytes from these mice were used to generate hybridomas. Two MAbs, designated 2B9A10 and 4C16B15 (of IgG1 and IgG3 isotypes, respectively), were subsequently selected and produced. They specifically recognized and precipitated recombinant hTPO produced by mammalian cells and were effective in sandwich enzyme-linked immunosorbent assays (ELISAs) for hTPO quantitation. Our results demonstrate that these MAbs should be useful for purification and quantitation of hTPO in clinical and laboratory settings.-
dc.publisherMary Ann Liebertko
dc.titleMonoclonal anti-thrombopoietin antibodies generated by genetic immunization-
dc.title.alternativeMonoclonal anti-thrombopoietin antibodies generated by genetic immunization-
dc.typeArticle-
dc.citation.titleHybridoma-
dc.citation.number2-
dc.citation.endPage79-
dc.citation.startPage75-
dc.citation.volume25-
dc.contributor.alternativeName임남규-
dc.contributor.alternativeName김정환-
dc.contributor.alternativeName김세연-
dc.contributor.alternativeName강현정-
dc.contributor.alternativeName김근수-
dc.contributor.alternativeName이상윤-
dc.contributor.alternativeName홍효정-
dc.contributor.alternativeName인경수-
dc.identifier.bibliographicCitationHybridoma, vol. 25, no. 2, pp. 75-79-
dc.identifier.doi10.1089/hyb.2006.25.75-
dc.description.journalClassN-
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