Characterization of an extracellular xylanase in Panibacillus sp. HY-8 isolated from an herbivorous longicorn beetle = 털두꺼비하늘소로부터 분리한 Panibacillus sp. HY-8이 생산하는 자일라나제의 특성

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Title
Characterization of an extracellular xylanase in Panibacillus sp. HY-8 isolated from an herbivorous longicorn beetle = 털두꺼비하늘소로부터 분리한 Panibacillus sp. HY-8이 생산하는 자일라나제의 특성
Author(s)
Sunyeon heo; Jangryul Kwak; Hyun Woo OhDoo Sang Park; Kyung Sook Bae; D H Shin; Ho Yong Park
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 16, no. 11, pp. 1753-1759
Publication Year
2006
Abstract
Paenibacillus sp. HY-8 isolated from the digestive tracts of the longicorn beetle, Moechotypa diphysis, produced an extracellular endoxylanase with a molecular weight of 20 kDa estimated by SDS-PAGE. The xylanase was purified to near electrophoretic homogeneity from the culture supernatant after ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography. The purified xylanase exhibited the highest activities at pH 6.0 and 50°C. The Km and Vmax values were 7.2 mg/ml and 16.3 U/mg, respectively, for birchwood xylan as the substrate. Nucleotide sequence of the PCR-cloned gene was determined to have the open reading frame encoding a polypeptide of 212 amino acids. The N-terminal amino acid sequence and the nucleotide sequence analyses predicted that the precursor xylanase contained a signal peptide composed of 28 amino acids and a catalytically active 19.9-kDa peptide fragment. The deduced amino acid sequence shared extensive similarity with those of the glycoside hydrolase family 11 of xylanases from other bacteria. The predicted amino acid sequence contained two glutamate residues, previously identified as essential and conserved for active sites in other xylanases of the glycoside hydrolase family 11.
Keyword
paenibacilluspurificationxylanxylanasebeetle
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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