Brevundimonas terrae sp. nov., isolated from an alkaline soil in Korea

Abstract

A Gram-negative, rod-shaped, Brevundimonas-like bacterial strain, KSL-145T, was isolated from an alkaline soil in Korea and subjected to a polyphasic taxonomic investigation. Strain KSL-145T grew optimally at pH 7.5-8.0 and 30 °C without NaCl. It was characterized chemotaxonomically as containing Q-10 as the predominant ubiquinone and C18:1 ω7c and C16:0 as the major fatty acids. The DNA G+C content was 61.8 mol%. A phylogenetic analysis based on 16S RNA gene sequences showed that strain KSL-145T fell within the radiation of the cluster comprising Brevundimonas species and Mycoplana bullata. The levels of 16S RNA gene sequence similarity between strain KSL-145T and the type strains of Brevundimonas species and M. bullata ranged from 95.3 to 98.7%. The mean DNA-DNA relatedness values between strain KSL-145T and the type strains of Brevundimonas diminuta and M. bullata, the closest phylogenetic relatives, were 26 and 15%, respectively. Strain KSL-145T could be differentiated from Brevundimonas species and M. bullata by differences in several phenotypic characteristics. On the basis of the phenotypic, phylogenetic and genetic data, strain KSL-145T represents a novel species in the genus Brevundimonas, for which the name Brevundimonas terrae sp. nov. is proposed. The type strain is KSL-145T (= KCTC 1248T=JCM 13476T.