Inhibitory effect of Thujae orientalis semen extract on pancreatic lipase activity = 백자인 추출물에 의한 pancreatic lipase의 저해 효과

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Title
Inhibitory effect of Thujae orientalis semen extract on pancreatic lipase activity = 백자인 추출물에 의한 pancreatic lipase의 저해 효과
Author(s)
Min-Soo KimBo Yeon Kim; Chan Sun Park; Byung Dae Yoon; S C Ahn; Won Keun Oh; Jong Seog Ahn
Bibliographic Citation
Korean Journal of Life Sciences, vol. 16, no. 2, pp. 328-332
Publication Year
2006
Abstract
The possible presence of inhibitors of pancreatic lipase (tricaylglycerol acylhydrolase EC 3.1.1.3) was screened from Korean traditional edible or medicinal herbs. Among tested herbs, Arecae pericarpium, Mucunae Caulis, Rhus javanica, Thujae orientalis were shown to have strong inhibitory effect against pancreatic lipase. Thujae orientalis was finally selected as a candidate for pancreatic lipase inhibitor. The extract of Thujae orientalis was showed selective inhibition on porcine pancreatic lipase activity. Active inhibitors, TF-1, TF-2, TF-3, were purified from an extract of Thujae orientalis, using chloroform extraction, followed by successive chromatography in silica gel and LH-20 and high performance liquid chromatography (HPLC). The IC50 values of TF-1, TF-2, TF-3 and orlistat were 44.7, 98.7, 46.1 and 27.6 ㎍/ml, respectively. And also the TF-2 and orlistat were shown to be inhibitory effect on the differentiation of preadipocyte NIH-3T3 L1 cells at a concentration of 10 ㎍/ml.
Keyword
pancreatic lipase inhibitorthujae orientalis
ISSN
I000-0052
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5352/JLS.2006.16.2.328
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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