Use of paromomycin as a selectable marker for the transformation of chinese cabbage = 배추의 형질전환용 선발항생제로서 paromomycin의 이용

Abstract

Hypocotyl explants of Chinese cabbage (cvs. “Jeong Sang” and “Seoul”) produced adventitious shoots on Murashige and Skoog (MS) basal medium supplemented with 4 mg/L AgNO3, 5 mg/L acetosyringone, 4 mg/L 6-benzyladenine and 3 mg/L alpha-naphthaleneacetic acid (SI) after cocultivation with strains of Agrobacterium tumefaciens (LBA4404) harboring the pCAMBIA1301 and the pPTN290 containing hygromycinresistance gene and paromomycin-resistance gene as a selectable marker genes, respectively. There was a significant difference in the frequency of transgenic plants depending on antibiotics and cultivars used. Paromomycin was better than hygromycin, and cultivar “Jeong-sang” was higher than “c.v. Seoul” in the frequency of transgenic plants. In particular, the highest frequency (0.70%) of transgenic plants was obtained from selection medium (SI) containing 100 mg/L paromomycin in c.v., “Jeong-sang”. GUS positive response were obtained 9 plants and 3 plants from the cultivars, “Jeong-sang” and “Seoul”, respectively. They were grown to maturity in a greenhouse and normally produced T1 seeds. GUS histochemical assay for progeny (T1) revealed that the transgenes were expressed in the plant genome.