Production of human serum albumin in chloroplast-transformed tobacco plants

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Title
Production of human serum albumin in chloroplast-transformed tobacco plants
Author(s)
S M Ko; H C Kim; B H Yoo; J W Yoo; Hwa-Jee Chung; D W Choi; Jang Ryol Liu
Bibliographic Citation
Journal of Plant Biotechnology, vol. 33, no. 4, pp. 233-236
Publication Year
2006
Abstract
Human serum albumin (HSA) is the most abundant protein in plasma and is the most often used intravenous protein in many human therapies. However, HSA is currently extracted only from plasma because commercially feasible recombinant expression systems are not available. This study attempted to develop an efficient system for recombinant HSA production by chloroplast transformation of tobacco. A HSA cDNA was isolated from a cDNA library constructed with human liver tissue. Chloroplast transformation vectors were constructed by introducing various regulatory elements to HSA regulatory sequences. Vectors were delivered by particle bombardment into leaf explants and chloroplasttransformed plants were subsequently regenerated into whole plants. Southern blot analysis confirmed that the HSA cDNA was incorporated between rps12 and orf70B of the chloroplast genome as designed. Western blot analysis revealed that hyper-expression and increasing the stability of HSA were achieved by modification of the regulatory sequences using the psbA5'UTRs in combination with elements of the 14 N-terminal amino acids of the GFP and the FLAG tag. However, only plants transformed with the vector containing all of these elements were able to accumulate HSA.
ISSN
1229-2818
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2006.33.4.233
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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