Halobacillus blutaparonensis sp. nov., a moderately halophilic bacterium isolated from Blutaparon portulacoides roots in Brazil = 브라질에서 분리한 신종 Halobacillus blutaparonensis

Abstract

A moderately halophilic, Gram-positive, sporeforming bacterium was isolated from the roots of Blutaparon portulacoides, a plant found in sandy soil parallel to the beach line in Restinga de Jurubatiba, Rio de Janeiro, Brazil. The strain, designated M9T, was motile and strictly aerobic with rod-shaped cells. It grew in the absence of NaCl and up to 20% NaCl, and was able to hydrolyze casein and starch. Strain M9T had a cell-wall peptidoglycan based on L-Orn-D-Asp, the predominant menaquinone present was menaquinone-7 (MK-7), diaminopimelic acid was not found, and anteiso-C15:0 and iso-C15:0 were the major fatty acids. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain M9T belonged to the genus Halobacillus and exhibited 16S rRNA gene similarity levels of 97.8-99.4% with the type strains of the other nine Halobacillus species. The DNA-DNA relatedness of strain M9T with H. trueperi, the closest relative as regards 16S rRNA gene similarity, and H. locisalis was 21 % and 18%, respectively. Therefore, on the basis of phenotypic, genotypic, and phylogenetic data, strain M9T (=ATCC BAA-1217T, =CIP 108771T, =KCTC 3980T) should be placed in the genus Halobacillus as a member of a novel species, for which the name Halobacillus blutaparonensis sp. nov. is proposed.