Marinobacterium halophilum sp. nov., a marine bacterium isolated from the Yellow Sea = 신종 Marinobacterium halophilum

Abstract

A moderately halophilic, aerobic, Gram-negative bacterium was isolated from a tidal flat area of Dae-Chun, Chung-Nam, Korea. The strain, designated mano11T, comprised rod-shaped cells that were motile by means of polar flagella. It grew with 3-12% NaCl and at 4-37 °C and pH 5.3-9.3. The predominant menaquinone present in this strain was MK-7 and diaminopimelic acid was not found in the cell-wall peptidoglycan. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain mano11T belongs to the genus Marinobacterium. Strain manol1T exhibited 92.8-98.3% 16S rRNA gene sequence similarity when compared with the type strains of three other species of the genus Marinobacterium. DNA-DNA hybridization between strain mano11T and Maninobacterium georgiense DSM 11526T, its closest relative in terms of 16S rRNA gene sequence similarity, was 13%. On the basis of the phenotypic, genetic and phylogenetic data, strain mano11T represents a novel species of the genus Marinobacterium, for which the name Marinobacterium halophilum sp. nov. is proposed. The type strain is mano11T (= KCTC 12240T = DSM 17586T).