Expression of thermostable α-glucosidase from Thermus caldophilus GK24 in recombinant Saccharomyces cerevisiae

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dc.contributor.authorJ Y Choi-
dc.contributor.authorJungoh Ahn-
dc.contributor.authorS I Kim-
dc.contributor.authorH J Shin-
dc.date.accessioned2017-04-19T09:06:33Z-
dc.date.available2017-04-19T09:06:33Z-
dc.date.issued2006-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7780-
dc.description.abstractA gene (GenBank AF096282) coding for a α-glucosidase (TcaAG, EC 3.2.1.20) from Thermus caldophilus GK24 was expressed in Saccharomyces cerevisiae, a generally recognized as safe (GRAS) host. The thermostable α-glucosidase was produced inside of the GRAS host at 0.04 unit/ mg-dry cell by the constitutively expressing ADH1 promoter and at 1.2 unit/ mg-dry cell by the inductively expressing GAL10 promoter, respectively. No α-glucosidase activities were found in the medium when the MF-alpha signal sequence from S. cerevisiae or α-amylase signal sequence from Aspergillus oryzae were fused before the α-glucosidase gene for the secretion.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleExpression of thermostable α-glucosidase from Thermus caldophilus GK24 in recombinant Saccharomyces cerevisiae-
dc.title.alternativeExpression of thermostable α-glucosidase from Thermus caldophilus GK24 in recombinant Saccharomyces cerevisiae-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number12-
dc.citation.endPage2003-
dc.citation.startPage2000-
dc.citation.volume16-
dc.contributor.affiliatedAuthorJungoh Ahn-
dc.contributor.alternativeName최재열-
dc.contributor.alternativeName안정오-
dc.contributor.alternativeName김선일-
dc.contributor.alternativeName신현재-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 16, no. 12, pp. 2000-2003-
dc.subject.keywordα-Glucosidase-
dc.subject.keywordprotein expression-
dc.subject.keywordrecombinant enzyme-
dc.subject.keywordsaccharomyces cerevisiae-
dc.subject.keywordthermus caldosphilus Gk24-
dc.subject.localAlpha-glucosidase-
dc.subject.localalpha-glucosidases-
dc.subject.localα-Glucosidase-
dc.subject.localα-glucosidase-
dc.subject.localProtein expression-
dc.subject.localprotein expression-
dc.subject.localProtein Expression-
dc.subject.localrecombinant enzyme-
dc.subject.localRecombinant enzyme-
dc.subject.localSaccharomyces cerevisiae-
dc.subject.localsaccharomyces cerevisiae-
dc.subject.localThermus caldophilus GK-24-
dc.subject.localThermus caldophilus GK24-
dc.subject.localthermus caldophilus GK-24-
dc.subject.localthermus caldophilus GK24-
dc.subject.localthermus caldosphilus Gk24-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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