DC Field | Value | Language |
---|---|---|
dc.contributor.author | J Y Choi | - |
dc.contributor.author | Jungoh Ahn | - |
dc.contributor.author | S I Kim | - |
dc.contributor.author | H J Shin | - |
dc.date.accessioned | 2017-04-19T09:06:33Z | - |
dc.date.available | 2017-04-19T09:06:33Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7780 | - |
dc.description.abstract | A gene (GenBank AF096282) coding for a α-glucosidase (TcaAG, EC 3.2.1.20) from Thermus caldophilus GK24 was expressed in Saccharomyces cerevisiae, a generally recognized as safe (GRAS) host. The thermostable α-glucosidase was produced inside of the GRAS host at 0.04 unit/ mg-dry cell by the constitutively expressing ADH1 promoter and at 1.2 unit/ mg-dry cell by the inductively expressing GAL10 promoter, respectively. No α-glucosidase activities were found in the medium when the MF-alpha signal sequence from S. cerevisiae or α-amylase signal sequence from Aspergillus oryzae were fused before the α-glucosidase gene for the secretion. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Expression of thermostable α-glucosidase from Thermus caldophilus GK24 in recombinant Saccharomyces cerevisiae | - |
dc.title.alternative | Expression of thermostable α-glucosidase from Thermus caldophilus GK24 in recombinant Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.number | 12 | - |
dc.citation.endPage | 2003 | - |
dc.citation.startPage | 2000 | - |
dc.citation.volume | 16 | - |
dc.contributor.affiliatedAuthor | Jungoh Ahn | - |
dc.contributor.alternativeName | 최재열 | - |
dc.contributor.alternativeName | 안정오 | - |
dc.contributor.alternativeName | 김선일 | - |
dc.contributor.alternativeName | 신현재 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, vol. 16, no. 12, pp. 2000-2003 | - |
dc.subject.keyword | α-Glucosidase | - |
dc.subject.keyword | protein expression | - |
dc.subject.keyword | recombinant enzyme | - |
dc.subject.keyword | saccharomyces cerevisiae | - |
dc.subject.keyword | thermus caldosphilus Gk24 | - |
dc.subject.local | Alpha-glucosidase | - |
dc.subject.local | alpha-glucosidases | - |
dc.subject.local | α-Glucosidase | - |
dc.subject.local | α-glucosidase | - |
dc.subject.local | Protein expression | - |
dc.subject.local | protein expression | - |
dc.subject.local | Protein Expression | - |
dc.subject.local | recombinant enzyme | - |
dc.subject.local | Recombinant enzyme | - |
dc.subject.local | Saccharomyces cerevisiae | - |
dc.subject.local | saccharomyces cerevisiae | - |
dc.subject.local | Thermus caldophilus GK-24 | - |
dc.subject.local | Thermus caldophilus GK24 | - |
dc.subject.local | thermus caldophilus GK-24 | - |
dc.subject.local | thermus caldophilus GK24 | - |
dc.subject.local | thermus caldosphilus Gk24 | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.