Donghicola eburneus gen. nov., sp. nov., isolated from seawater of the East Sea in Korea

Abstract

A Gram-negative, aerobic, slightly halophilic, non-motile and coccoid- or rod-shaped bacterial strain, SW-277T was isolated from seawater, Korea, and subjected to a polyphasic taxonomic study. Strain SW-277T was catalase- and oxidase-positive and grew optimally at pH 7.0-8.0 and 37 °C in the presence of 2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SW-277T belongs to the Alphaproteobacteria as part of the clade comprising the genera Roseivivax and Salipiger with a bootstrap resampling value of 72.5%. 16S rRNA gene sequence similarity values between strain SW-277T and members of the genera Roseivivax and Salipiger were between 93.8 and 94.7%. Strain SW-277T contained Q-10 as the predominant ubiquinone and C18:1ω7c and C16:0 as the major fatty acids. The DNA G + C content was 59.7 mol%. Strain SW-277T could be distinguished from members of the phylogenetically related genera Salipiger and Roseivivax by phenotypic differences and low 16S rRNA gene similarity values. On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic data, strain SW-277T (=KCTC 12735T = JCM 13604T) should be classified as a representative of a novel species in a new genus, Donghicola eburneus gen. nov., sp. nov.