Equol inhibits nitric oxide production and inducible nitric oxide synthase gene expression through down-regulating the activation of Akt

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Title
Equol inhibits nitric oxide production and inducible nitric oxide synthase gene expression through down-regulating the activation of Akt
Author(s)
Jong Soon KangYeo Dae Yoon; Mi Hwa Han; Sang Bae Han; Kiho Lee; Song Kyu Park; Hwan Mook Kim
Bibliographic Citation
International Immunopharmacology, vol. 7, no. 4, pp. 491-499
Publication Year
2007
Abstract
In the present study, we report the inhibitory effect of equol on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) gene expression in murine macrophages. In vivo administration of equol (i.p.) attenuated NO production by peritoneal adherent cells isolated from lipopolysaccharide (LPS)-treated mice. Equol dose-dependently inhibited the LPS-induced production of NO in isolated peritoneal adherent cells and RAW 264.7 cells. The mRNA expression of iNOS was also blocked by equol in LPS-stimulated RAW 264.7 cells. Further study demonstrated that the LPS-induced activation of Akt was suppressed by equol in RAW 264.7 cells while the activation of ERK, SAPK/JNK and p38 MAP kinase was not affected. Equol also blocked LPS-induced NF-κB activation. Moreover, the LPS-induced NO production and NF-κB activation was inhibited by LY294002, a specific inhibitor of phosphatidylinositol 3-kinase/Akt pathway, in RAW 264.7 cells. These results suggest that equol might inhibit NO production and iNOS gene expression, at least in part, by blocking Akt activation and subsequent down-regulation of NF-κB activity.
Keyword
AktEquolInducible nitric oxide synthaseNF-κBNitric oxide
ISSN
1567-5769
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.intimp.2006.12.004
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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