Interaction proteome analysis of Xanthomonas Hrp proteins

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Title
Interaction proteome analysis of Xanthomonas Hrp proteins
Author(s)
Mi Jang; Byoung Chul Park; Do Hee Lee; Kwang-Hee Bae; S Cho; H S Park; B R Lee; Sung Goo Park
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 17, no. 2, pp. 359-363
Publication Year
2007
Abstract
Because of the importance of the type III protein-secretion system in bacteria-plant interaction, its function in bacterial pathogenesis of plants has been intensively studied. To identify bacterial proteins interacting with Xanthomonas hrp gene products that are involved in pathogenicity, we performed the glutathione-bead binding analysis of Xanthomonas lysates containing GST-tagged Hrp proteins. Analysis of glutathione-bead bound proteins by 1-DE and MALDI-TOF has demonstrated that Avr proteins, RecA, and several components of the type III secretion system interact with HrpB protein. This proteomic approach could provide a powerful tool in finding interaction partners of Hrp proteins whose roles in host-pathogen interaction need further studies.
Keyword
1-DEhrp geneinteraction proteinMALDITOFpathogenesis
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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