DC Field | Value | Language |
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dc.contributor.author | Yong Beom Shin | - |
dc.contributor.author | Jeong-Min Lee | - |
dc.contributor.author | M R Park | - |
dc.contributor.author | Min-Gon Kim | - |
dc.contributor.author | Bong Hyun Chung | - |
dc.contributor.author | H B Pyo | - |
dc.contributor.author | S Maeng | - |
dc.date.accessioned | 2017-04-19T09:06:46Z | - |
dc.date.available | 2017-04-19T09:06:46Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | 10.1016/j.bios.2006.12.028 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7829 | - |
dc.description.abstract | The localized surface plasmon resonance (LSPR)-based optical biosensor using nano-structures of noble metals has been considered as a useful tool for label-free detection of DNA hybridization and protein-protein interactions. We fabricated LSPR-based optical biosensors using gold nano-islands (nominal thickness; 75 ?) on glass substrates that were easily made using the conventional fabrication methods. The formation of gold nano-islands on glass substrates was realized by heat treatment of thin gold film deposited with a low deposition rate (∼0.05 ?/s). The morphologies of sensor surfaces composed of gold nano-islands were observed using an atomic force microscope (AFM) with a non-contact mode. To investigate the sensing capacity of the gold nano-island sensor for the binding of proteins by affinity interactions, the streptavidin and biotin interaction was used as a model system. In addition, detection of recombinant glutathione-S-transferase (GST)-tagged human interleukin-6 (hIL6) expressed in Escherichia coli was carried out by LSPR. It is expected that the LSPR sensors composed of gold nano-islands can be an alternative to traditional methods such as SDS-polyacrylamide gel electrophoresis (SDS-PAGE) for fast analysis of protein expression. | - |
dc.publisher | Elsevier | - |
dc.title | Analysis of recombinant protein expression using localized surface plasmon resonance (LSPR) | - |
dc.title.alternative | Analysis of recombinant protein expression using localized surface plasmon resonance (LSPR) | - |
dc.type | Article | - |
dc.citation.title | Biosensors & Bioelectronics | - |
dc.citation.number | 9 | - |
dc.citation.endPage | 2307 | - |
dc.citation.startPage | 2301 | - |
dc.citation.volume | 22 | - |
dc.contributor.affiliatedAuthor | Yong Beom Shin | - |
dc.contributor.affiliatedAuthor | Jeong-Min Lee | - |
dc.contributor.affiliatedAuthor | Min-Gon Kim | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.alternativeName | 신용범 | - |
dc.contributor.alternativeName | 이정민 | - |
dc.contributor.alternativeName | 박미라 | - |
dc.contributor.alternativeName | 김민곤 | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.contributor.alternativeName | 표현봉 | - |
dc.contributor.alternativeName | 맹성렬 | - |
dc.identifier.bibliographicCitation | Biosensors & Bioelectronics, vol. 22, no. 9, pp. 2301-2307 | - |
dc.identifier.doi | 10.1016/j.bios.2006.12.028 | - |
dc.subject.keyword | Affinity-tagged protein | - |
dc.subject.keyword | Attenuated total reflection | - |
dc.subject.keyword | Gold nano-island | - |
dc.subject.keyword | Label-free assay | - |
dc.subject.keyword | Localized surface plasmon resonance (LSPR) | - |
dc.subject.keyword | Well chip | - |
dc.subject.local | Affinity-tagged protein | - |
dc.subject.local | attenuated total reflection (ATR) | - |
dc.subject.local | Attenuated total reflection | - |
dc.subject.local | Gold nano-island | - |
dc.subject.local | gold nano-island | - |
dc.subject.local | Label-free assay | - |
dc.subject.local | localized surface plasmon resonance | - |
dc.subject.local | Localized surface plasmon resonance (LSPR) | - |
dc.subject.local | Localized surface plasmon resonance | - |
dc.subject.local | localized surface plasmon resonance (LSPR) | - |
dc.subject.local | Well chip | - |
dc.subject.local | well chip | - |
dc.description.journalClass | Y | - |
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