Analysis of recombinant protein expression using localized surface plasmon resonance (LSPR)

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dc.contributor.authorYong Beom Shin-
dc.contributor.authorJeong-Min Lee-
dc.contributor.authorM R Park-
dc.contributor.authorMin-Gon Kim-
dc.contributor.authorBong Hyun Chung-
dc.contributor.authorH B Pyo-
dc.contributor.authorS Maeng-
dc.date.accessioned2017-04-19T09:06:46Z-
dc.date.available2017-04-19T09:06:46Z-
dc.date.issued2007-
dc.identifier.issn0956-5663-
dc.identifier.uri10.1016/j.bios.2006.12.028ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7829-
dc.description.abstractThe localized surface plasmon resonance (LSPR)-based optical biosensor using nano-structures of noble metals has been considered as a useful tool for label-free detection of DNA hybridization and protein-protein interactions. We fabricated LSPR-based optical biosensors using gold nano-islands (nominal thickness; 75 ?) on glass substrates that were easily made using the conventional fabrication methods. The formation of gold nano-islands on glass substrates was realized by heat treatment of thin gold film deposited with a low deposition rate (∼0.05 ?/s). The morphologies of sensor surfaces composed of gold nano-islands were observed using an atomic force microscope (AFM) with a non-contact mode. To investigate the sensing capacity of the gold nano-island sensor for the binding of proteins by affinity interactions, the streptavidin and biotin interaction was used as a model system. In addition, detection of recombinant glutathione-S-transferase (GST)-tagged human interleukin-6 (hIL6) expressed in Escherichia coli was carried out by LSPR. It is expected that the LSPR sensors composed of gold nano-islands can be an alternative to traditional methods such as SDS-polyacrylamide gel electrophoresis (SDS-PAGE) for fast analysis of protein expression.-
dc.publisherElsevier-
dc.titleAnalysis of recombinant protein expression using localized surface plasmon resonance (LSPR)-
dc.title.alternativeAnalysis of recombinant protein expression using localized surface plasmon resonance (LSPR)-
dc.typeArticle-
dc.citation.titleBiosensors & Bioelectronics-
dc.citation.number9-
dc.citation.endPage2307-
dc.citation.startPage2301-
dc.citation.volume22-
dc.contributor.affiliatedAuthorYong Beom Shin-
dc.contributor.affiliatedAuthorJeong-Min Lee-
dc.contributor.affiliatedAuthorMin-Gon Kim-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName신용범-
dc.contributor.alternativeName이정민-
dc.contributor.alternativeName박미라-
dc.contributor.alternativeName김민곤-
dc.contributor.alternativeName정봉현-
dc.contributor.alternativeName표현봉-
dc.contributor.alternativeName맹성렬-
dc.identifier.bibliographicCitationBiosensors & Bioelectronics, vol. 22, no. 9, pp. 2301-2307-
dc.identifier.doi10.1016/j.bios.2006.12.028-
dc.subject.keywordAffinity-tagged protein-
dc.subject.keywordAttenuated total reflection-
dc.subject.keywordGold nano-island-
dc.subject.keywordLabel-free assay-
dc.subject.keywordLocalized surface plasmon resonance (LSPR)-
dc.subject.keywordWell chip-
dc.subject.localAffinity-tagged protein-
dc.subject.localattenuated total reflection (ATR)-
dc.subject.localAttenuated total reflection-
dc.subject.localGold nano-island-
dc.subject.localgold nano-island-
dc.subject.localLabel-free assay-
dc.subject.locallocalized surface plasmon resonance-
dc.subject.localLocalized surface plasmon resonance (LSPR)-
dc.subject.localLocalized surface plasmon resonance-
dc.subject.locallocalized surface plasmon resonance (LSPR)-
dc.subject.localWell chip-
dc.subject.localwell chip-
dc.description.journalClassY-
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Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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