Translation elongation factor 1-α gene from Pichia pastoris: Molecular cloning, sequence, and use of its promoter

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Title
Translation elongation factor 1-α gene from Pichia pastoris: Molecular cloning, sequence, and use of its promoter
Author(s)
Jungoh Ahn; Jiyeon Hong; Hyeok Won Lee; Myongsoo Park; Eun Gyo Lee; Chun Sug Kim; Eui Sung Choi; Joonki Jung; Hong-Weon Lee
Bibliographic Citation
Applied Microbiology and Biotechnology, vol. 74, no. 3, pp. 601-608
Publication Year
2007
Abstract
The gene encoding translation elongation factor 1-α from the yeast Pichia pastoris was cloned. The gene revealed an open reading frame of 1,380 bp with the potential to encode a polypeptide of 459 amino acids with a calculated mass of 50.1 kDa. The potential of the promoter (P TEF1 ) in P. pastoris was investigated with comparison to the glyceraldehyde-3-phosphate dehydrogenase promoter (P GAP ) by using a bacterial lipase gene as a reporter gene. P TEF1 demonstrated a tighter growth-associated expression mode, improved functioning in the presence of high glucose concentrations, and promoter activities that yielded recombinant protein at levels similar to or in one case greater than P GAP . The sequence of the gene was deposited in GenBank under accession no. EF014948.
Keyword
constitutive promoterpichia pastoristranslation elongation factor
ISSN
0175-7598
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00253-006-0698-6
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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