Identification of a fibrinolytic enzyme by Bacillus vallismortis and its potential as a bacteriolytic enzyme against Streptococcus mutans

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Title
Identification of a fibrinolytic enzyme by Bacillus vallismortis and its potential as a bacteriolytic enzyme against Streptococcus mutans
Author(s)
J B Kim; W H Jung; J M Ryu; Y J Lee; Joon-Ki Jung; H W Jang; S W Kim
Bibliographic Citation
Biotechnology Letters, vol. 29, no. 4, pp. 605-610
Publication Year
2007
Abstract
A potent fibrinolytic enzyme-producing bacterium was isolated from the traditional Korean condiment Chungkook-jang and identified as Bacillus vallismortis Ace02. The extracellular fibrinolytic enzyme was purified with a 18% recovery of activity from supernatant cultures using CM-Sepharose column chromatography and Sephacryl S-200 gel filtration. The specific activity of the purified enzyme was 757 kFU mg-1. Its molecular mass was about 28 kDa and the initial amino acids of the N-terminal sequence were AQSVPYGVSQ. The full amino acid sequence of fibrinolytic enzyme Ace02 corresponded with bacteriolytic enzyme, L27, from Bacillus licheniformis, which has strong lytic activity against Streptococcus mutans, a major causative strain of dental caries. This suggests that the purified enzyme should be used for prevention of dental caries as well as being an effective thrombolytic agent.
Keyword
Bacteriolytic enzymeFibrinolytic enzymeBacillus vallismortis Ace02
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-006-9284-3
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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