Identification of genes related to Parkinson's disease using expressed sequence tags

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Title
Identification of genes related to Parkinson's disease using expressed sequence tags
Author(s)
J M Kim; Kyu-Hwa Lee; Yeo-Jin Jeon; J H Oh; So-Young Jeong; In Sung Song; J M Kim; D S Lee; Nam-Soon Kim
Bibliographic Citation
DNA Research, vol. 13, no. 6, pp. 275-286
Publication Year
2006
Abstract
In a search for novel target genes related to Parkinson's disease (PD), two full-length cDNA libraries were constructed from a human normal substantia nigra (SN) and a PD patient's SN. An analysis of the gene expression profiles between them was done using the expressed sequence tags (ESTs) frequency. Data for the differently expressed genes were verified by quantitative real-time RT-PCR, immunohistochemical analysis and a cell death assay. Among the 76 genes identified with a significant difference (P > 0.9), 21 upregulated genes and 13 downregulated genes were confirmed to be differentially expressed in human PD tissues and/or in an MPTP-treated mice model by quantitative real-time RT-PCR. Among those genes, an immunohistochemical analysis using an MPTP mice model for alpha-tubulin including TUBA3 and TUBA6 showed that the protein levels are downregulated, as well as the RNA levels. In addition, MBP, PBP and GNAS were confirmed to accelerate cell death activity, whereas SPP1 and TUBA3 to retard this process. Using an analysis of ESTs frequency, it was possible to identify a large number of genes related to human PD. These new genes, MBP, PBP, GNAS, SPP1 and TUBA3 in particular, represent potential biomarkers for PD and could serve as useful targets for elucidating the molecular mechanisms associated with PD.
Keyword
Cell deathExpressed sequence tagsGene expression profilingImmunohistochemistryParkinson's disease
ISSN
1340-2838
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/dnares/dsl016
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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