DC Field | Value | Language |
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dc.contributor.author | Eun-Ju Jeong | - |
dc.contributor.author | Kyoungsook Park | - |
dc.contributor.author | Hyou-Arm Joung | - |
dc.contributor.author | Chang-Soo Lee | - |
dc.contributor.author | D W Seol | - |
dc.contributor.author | Bong Hyun Chung | - |
dc.contributor.author | Moonil Kim | - |
dc.date.accessioned | 2017-04-19T09:06:50Z | - |
dc.date.available | 2017-04-19T09:06:50Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | 10.1007/s10529-007-9313-x | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7858 | - |
dc.description.abstract | Conformational changes in hexokinase are induced by its binding to glucose, thus providing an excellent example of an 'induced fit' model. To observe glucose-induced fluorescence restoration in hexokinase II using split-enhanced, green fluorescent protein (EGFP) in a process involving the reconstitution of split EGFP, E. coli cells expressing the chimeric NEGFP:HXK:CEGFP recombinant protein were treated with glucose and visualized via fluorescence read-outs. The reconstituted EGFP generated a strong fluorescence upon glucose stimulation of the bacteria. Moreover, the fluorescence intensity became stronger with increasing glucose up to 10 mM, with a maximum being observed after 60 min in a time- and concentration-dependent manner. Conformational changes associated with glucose-induced fit in human hexokinase II can thus be monitored successfully in vivo via fluorescence reconstitution assays, coupled with a quick and easy fluorescent read-out protocol. | - |
dc.publisher | Springer | - |
dc.title | Detection of glucose-induced conformational change in hexokinase II using fluorescence complementation assay | - |
dc.title.alternative | Detection of glucose-induced conformational change in hexokinase II using fluorescence complementation assay | - |
dc.type | Article | - |
dc.citation.title | Biotechnology Letters | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 802 | - |
dc.citation.startPage | 797 | - |
dc.citation.volume | 29 | - |
dc.contributor.affiliatedAuthor | Eun-Ju Jeong | - |
dc.contributor.affiliatedAuthor | Kyoungsook Park | - |
dc.contributor.affiliatedAuthor | Chang-Soo Lee | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.affiliatedAuthor | Moonil Kim | - |
dc.contributor.alternativeName | 정은주 | - |
dc.contributor.alternativeName | 박경숙 | - |
dc.contributor.alternativeName | 정효암 | - |
dc.contributor.alternativeName | 이창수 | - |
dc.contributor.alternativeName | 설대우 | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.contributor.alternativeName | 김문일 | - |
dc.identifier.bibliographicCitation | Biotechnology Letters, vol. 29, no. 5, pp. 797-802 | - |
dc.identifier.doi | 10.1007/s10529-007-9313-x | - |
dc.subject.keyword | Conformational change | - |
dc.subject.keyword | Fluorescence complementation assay | - |
dc.subject.keyword | Glucose-induced fit | - |
dc.subject.keyword | Hexokinase II | - |
dc.subject.keyword | Split EGFP | - |
dc.subject.local | conformational change | - |
dc.subject.local | Conformational change | - |
dc.subject.local | Fluorescence complementation assay | - |
dc.subject.local | Glucose-induced fit | - |
dc.subject.local | Hexokinase II | - |
dc.subject.local | Split EGFP | - |
dc.description.journalClass | Y | - |
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