Transduced PEP-1-Grb7 fusion protein suppressed LPS-induced COX-2 expression

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Title
Transduced PEP-1-Grb7 fusion protein suppressed LPS-induced COX-2 expression
Author(s)
J J An; S Y Kim; S H Lee; D W Kim; H J Ryu; S I Yeo; S H Jang; H J Kwon; T Y Kim; Sang Chul LeeHaryoung Poo; S W Cho; K S Lee; J Park; W S Eum; S Y Choi
Bibliographic Citation
BMB Reports, vol. 40, no. 2, pp. 189-195
Publication Year
2007
Abstract
Although the incidence and severity of atopic dermatitis (AD) is steadily increasing at an alarming rate, its pathogenic mechanisms remain poorly understood yet. Recently, we found that the expression of Grb7 protein was markedly decreased in AD patients using proteomic analysis. In the present study, human Grb7 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-Grb7 fusion protein. The expressed and purified PEP-1-Grb7 fusion proteins transduced efficiently into skin cells in a time- and dose-dependent manner when added exogenously in culture media. Once inside the cells, the transduced PEP-1-Grb7 protein was stable for 48 h. In addition, transduced PEP-1-Grb7 fusion protein markedly increased cell viability in macrophage RAW 264.7 cells treated with LPS by inhibition of the COX-2 expression level. These results suggest that the PEP-1-Grb7 fusion protein can be used in protein therapy for inflammatory skin disorders, including AD.
Keyword
atopic dermatitis (AD)COX-2PEP-1-Grb7protein therapyprotein transduction
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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