Plant regeneration from zygotic embryos cultures of Lilium lancifolium Thunb. via bulblet formation = 참나리(Lilium lancifolium Thunb.) 접합자배로부터 소자구 형성을 통한 식물체 재생

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Title
Plant regeneration from zygotic embryos cultures of Lilium lancifolium Thunb. via bulblet formation = 참나리(Lilium lancifolium Thunb.) 접합자배로부터 소자구 형성을 통한 식물체 재생
Author(s)
K H Kim; Jang Ryol Liu; Suk Weon Kim
Bibliographic Citation
Journal of Plant Biotechnology, vol. 34, no. 1, pp. 25-29
Publication Year
2007
Abstract
Plant regeneration system from zygotic embryos (2n=24) of Lilium lancifolium Thunb. via bulblet formation was estabished. Zygotic embryos of Lilium lancifolium formed bulblets and somatic embryos simultaneously when they cultured on MS medium supplemented with low concentration of 2,4-D. The highest frequency of bulblet and somatic embryo formation from zygotic embryos of Lilium lancifolium was 66.7% and 56.7%, respectively. The frequency of bulblet and somatic embryo formation was decreased when they cultured on MS medium over than 1 mg/L of 2,4-D. To regenerate whole plants, somatic embryos formed on zygotic embryos were transferred to MS basal medium. However somatic embryos did not fully converted into plantlets. Further incubation in the light, elongated somatic embryos formed numerous bulblets at the base of somatic embryos. Upon transfer to MS basal medium, bulblets were successfully converted into plantlets after further 4 weeks of culture in the light. After acclimatization, plantets from bulblets were transferred to soil and grown to normal plants in growth chamber (approximately , 16/8h photo period, ) The chromosome analysis revealed that plants regenerated from zygotic embryos showed 2n=24. These results indicate that chromosome stability of source tissue is maintained during plant regeneration via bulblet formation
ISSN
1229-2818
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5010/JPB.2007.34.1.025
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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