Identification of Novel regulators of apoptosis using a high-throughput cell-based screen

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Title
Identification of Novel regulators of apoptosis using a high-throughput cell-based screen
Author(s)
K M Park; E Kang; Yeo-Jin Jeon; N Kim; Nam-Soon Kim; Hyang Sook Yoo; Young Il Yeom; Soo Jung Kim
Bibliographic Citation
Molecules and Cells, vol. 23, no. 2, pp. 170-174
Publication Year
2007
Abstract
High-throughput subcellular imaging is a powerful tool for investigating the function of genes. In order to identify novel regulators of apoptosis we transiently transfected HeLa cells with 938 hypothetical genes of unknown function, and captured their nuclear images with an automated fluorescence microscope. We selected genes that induced greater than 3-fold increase in the percentage of apoptotic nuclei compared with vector-transfected cells. The full-length genes C10orf61, MGC 26717, and FLJ13855 were identified as candidate proapoptotic genes, and their apoptotic effects were confirmed by DNA fragmentation ELISAs and Western blotting for caspase-7 and PARP. We conclude that a subcellular image-based apoptotic screen is useful for identifying genes with proapoptotic activity.
Keyword
apoptosisautomated fluorescence microscopehypothetical genessubcellular imaging
ISSN
1016-8478
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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