Aeromicrobium panaciterrae sp. nov., isolated from soil of a ginseng field in South Korea

Abstract

A Gram-positive, rod-shaped, non-spore-forming and strictly aerobic bacterium (Gsoil 161T) was isolated from soil of a ginseng field in Pocheon Province, South Korea. The novel isolate was characterized using a polyphasic approach in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 161T was shown to belong to the family Nocardioidaceae and was related to Aeromicrobium marinum (98.0% similarity to the type strain), Aeromicrobium alkaliterrae (97.6%), Aeromicrobium fastidiosum (97.0%) and Aeromicrobium erythreum (96.7%); the sequence similarity with other species within the family was less than 94.4%. It was characterized chemotaxonomically as having LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-9(H4) as the predominant menaquinone and C16:0, 10-methyl C18:0 (tuberculostearic acid), C16:0 2-OH, 10-methyl C17:0 and 10-methyl-C16:0 as the major fatty acids. The G + C content of the genomic DNA was 65.5 mol%. These chemotaxonomic properties and phenotypic characteristics support the affiliation of strain Gsoil 161T to the genus Aeromicrobium. Results of physiological and biochemical tests enabled strain Gsoil 161T to be differentiated genotypically and phenotypically from currently known Aeromicrobium species. Therefore, strain Gsoil 161T represents a novel species, for which the name Aeromicrobium panaciterrae sp. nov. is proposed. The type strain is strain Gsoil 161T (=KCTC 19131T = DSM 17939T=CCUG 52476T).