Metagenomic analysis of BTEX-contaminated forest soil microcosm

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Title
Metagenomic analysis of BTEX-contaminated forest soil microcosm
Author(s)
Sang Chun Ji; D Kim; Jung-Hoon Yoon; C H Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 17, no. 4, pp. 668-672
Publication Year
2007
Abstract
A microcosmal experiment using a metagenomic technique was designed to assess the effect of BTEX (benzene, toluene, ethylbenzene, and xylenes) on an indigenous bacterial community in a Daejeon forest soil. A compositional shift of bacterial groups in an artificial BTEX-contaminated soil was examined by the 16S rDNA PCR-DGGE method. Phylogenetic analysis of 16S rDNAs in the dominant DGGE bands showed that the number of Actinobacteria and Bacillus populations increased. To confirm these observations, we performed PCR to amplify the 23S rDNA and 16S rDNA against the sample metagenome using Actinobacteria-targeting and Bacilli-specific primer sets, respectively. The result further confirmed that a bacterial community containing Actinobacteria and Bacillus was affected by BTEX.
Keyword
16S rDNA PCR-DGGEBTEXclass-specific PCR
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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