DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kyoung Sil Yang | - |
dc.contributor.author | H S Kim | - |
dc.contributor.author | U H Jin | - |
dc.contributor.author | S S Lee | - |
dc.contributor.author | J A Park | - |
dc.contributor.author | Y P Lim | - |
dc.contributor.author | H S Pai | - |
dc.date.accessioned | 2017-04-19T09:07:04Z | - |
dc.date.available | 2017-04-19T09:07:04Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0032-0935 | - |
dc.identifier.uri | 10.1007/s00425-006-0453-3 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7899 | - |
dc.description.abstract | BTF3 (βNAC) was originally isolated as a general transcription factor required for RNA polymerase II-dependent transcription, and later found to be a β-subunit of nascent-polypeptide-associated complex that has been implicated in regulating protein localization during translation. In this study, virus-induced gene silencing of NbBTF3 encoding a Nicotiana benthamiana homolog of human BTF3 caused leaf yellowing and abnormal leaf morphology without altering the overall growth of the plant. The NbBTF3 gene is constitutively expressed and the NbBTF3-GFP fusion protein is primarily targeted to the nucleus. At the cellular level, downregulation of NbBTF3 expression reduced the chloroplast sizes and chlorophyll contents. The affected cells produced excessive amounts of reactive oxygen species. Furthermore, the transcript level of various plastid- and mitochondria-encoded genes was severely reduced in the NbBTF3-depleted leaf cells. These findings indicate that depletion of NbBTF3 activity preferentially affected development and/or physiology of chloroplasts and mitochondria in plants, possibly by hampering efficient translocation of the nascent organellar proteins into the organelles. | - |
dc.publisher | Springer | - |
dc.title | Silencing of NbBTF3 results in developmental defects and disturbed gene expression in chloroplasts and mitochondria of higher plants | - |
dc.title.alternative | Silencing of NbBTF3 results in developmental defects and disturbed gene expression in chloroplasts and mitochondria of higher plants | - |
dc.type | Article | - |
dc.citation.title | Planta | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 1469 | - |
dc.citation.startPage | 1459 | - |
dc.citation.volume | 225 | - |
dc.contributor.affiliatedAuthor | Kyoung Sil Yang | - |
dc.contributor.alternativeName | 양경실 | - |
dc.contributor.alternativeName | 김희승 | - |
dc.contributor.alternativeName | 진운호 | - |
dc.contributor.alternativeName | 이상숙 | - |
dc.contributor.alternativeName | 박종아 | - |
dc.contributor.alternativeName | 임용표 | - |
dc.contributor.alternativeName | 배현숙 | - |
dc.identifier.bibliographicCitation | Planta, vol. 225, no. 6, pp. 1459-1469 | - |
dc.identifier.doi | 10.1007/s00425-006-0453-3 | - |
dc.subject.keyword | leaf yellowing | - |
dc.subject.keyword | nuclear localization | - |
dc.subject.keyword | organellar RNA polymerases | - |
dc.subject.keyword | reactive oxygen species | - |
dc.subject.keyword | virus-induced gene silencing | - |
dc.subject.local | leaf yellowing | - |
dc.subject.local | Nuclear localization | - |
dc.subject.local | nuclear localization | - |
dc.subject.local | organellar RNA polymerases | - |
dc.subject.local | ROS | - |
dc.subject.local | Reactive Oxygen Species (ROS) | - |
dc.subject.local | Reactive oxidative species | - |
dc.subject.local | Reactive oxygen species | - |
dc.subject.local | Reactive oxygen species (ROS) | - |
dc.subject.local | reactive oxygen species | - |
dc.subject.local | reactive oxygen species (ROS) | - |
dc.subject.local | Reactive Oxygen Species | - |
dc.subject.local | Reactive oxygen species(ROS) | - |
dc.subject.local | virus-induced gene silencing | - |
dc.subject.local | VIGS | - |
dc.subject.local | Virus-induced gene silencing | - |
dc.subject.local | Virus-induced gene silencing (VIGS) | - |
dc.description.journalClass | Y | - |
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