Development of marker-free transgenic potato hairy roots using Agrobacterium rhizogenes

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Development of marker-free transgenic potato hairy roots using Agrobacterium rhizogenes
Jung Won Youm; Jae Heung Jeon; Mi Sun Kim; Jae Hyun Kim; Yoon Sik Kim; Han Na Kim; Hyouk Joung; B D Kim; Hyun Soon Kim
Bibliographic Citation
Horticulture Environment and Biotechnology, vol. 48, no. 2, pp. 135-141
Publication Year
Agrobascterium rhizogenes KCTC2744 and A. tumefaciens LBA4404 harboring the binary vector pBIl21 were used to transform potato plants. Transformation efficiency was monitored by detecting the β-glucuronidase (GUS) transcripts and the enzyme activity. The GUS expression of potato hairy roots regenerated by A. rhizogenes-mediated transformation was found to be approximately two-fold higher than that by A. tumefaciens-mediated transformation. All hairy roots were induced by A. rhizogenes on Murashige and Skoog medium without antibiotics. The hairy roots were transformed to pBI121 and pCAMbar-by A. rhizogenes KCTC2744. The GUS expression patterns, including hairy roots regeneration and staining characteristics, were investigated. No significant differences were observed between the general transgenic hairy roots and the selectable marker-free transgenic hairy roots. The polymerase chain reaction amplification and Southern blots confirmed a stable integration of foreign genes into all of the marker-free transgenic hairy roots. This is the first genetic engineering approach to the transgenic potato hairy roots genome without the use of a selectable marker gene.
antibioticsgene transformationβ-glucuronidase
Korea Soc-Assoc-Inst
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Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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