Survey of herbicide resistant oilseed rapes around the basin of rivers in Incheon harbor area = 인천항만 주변 하천유역의 제초제 저항성 유전자변형유채 조사

Abstract

Genetic contaminations caused by increased import of genetically modified organism (GMO) are of concern in our ecosystem. This study was conducted to investigate the intentional or unintentional release of three herbicide resistant oilseed rape events, RT73, T45 and MS8/RF3, which have already been approved for their consumption in Korea in 2005, around the basin of rivers in Incheon harbor area. Seven sites in Seunggi-river and one site in Kajwa-river were investigated for the possible genetic contamination and release of GM oilseed rapes in April 2006. Gene specific primers for CP4EPSPS, 35S promoter and nos terminator genes were designed to detect herbicide resistant oilseed rape events. Based on the PCR analysis, any GM oilseed rape was not discovered in the surveyed area. Since the GTS40-3-2 soybean contains CP4EPSPS, 35S promoter and nos terminator genes, it was compatibly used for detecting the three oilseed rape events as positive controls in the PCR analysis. The gene flows from GM oilseed rape to its relatives, leaf mustard or Chinese cabbage were not occurred in the surveyed areas. This study also suggested that the rubisco small subunit primer was compatible for leaf mustard and Chinese cabbage in the PCR analysis as a positive control.