DC Field | Value | Language |
---|---|---|
dc.contributor.author | D Kim | - |
dc.contributor.author | Hee Gu Lee | - |
dc.contributor.author | H Jung | - |
dc.contributor.author | S H Kang | - |
dc.date.accessioned | 2017-04-19T09:07:15Z | - |
dc.date.available | 2017-04-19T09:07:15Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.uri | 10.5012/bkcs.2007.28.5.783 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7928 | - |
dc.description.abstract | The immobilization of proteins and their molecular interactions on various polymer-modified glass substrates [i.e. 3-aminopropyltriethoxysilane (APTS), 3-glycidoxypropyltrimethoxysilane (GPTS), poly (ethylene glycol) diacrylate (PEG-DA), chitosan (CHI), glutaraldehyde (GA), 3-(trichlorosilyl)propyl methacrylate (TPM), 3'-mercaptopropyltrimethoxysilane (MPTMS), glycidyl methacrylate (GMA) and poly-l-lysine (PL).] for potential applications in a nanoarray protein chip at the single-molecule level was evaluated using prism-type dual-color total internal reflection fluorescence microscopy (dual-color TIRFM). A dual-color TIRF microscope, which contained two individual laser beams and a single high-sensitivity camera, was used for the rapid and simultaneous dual-color detection of the interactions and colocalization of different proteins labeled with different fluorescent dyes such as Alexa Fluor® 488, Qdot® 525 and Alexa Fluor® 633. Most of the polymer-modified glass substrates showed good stability and a relative high signal-to-noise (S/N) ratio over a 40-day period after making the substrates. The GPTS/CHI/GA-modified glass substrate showed a 13.5-56.3% higher relative S/N ratio than the other substrates. 1% Top-Block in 10 mM phosphate buffered saline (pH 7.4) showed a 99.2% increase in the blocking effect of non-specific adsorption. These results show that dual-color TIRFM is a powerful methodology for detecting proteins at the single-molecule level with potential applications in nanoarray chips or nano-biosensors. | - |
dc.publisher | Wiley | - |
dc.title | Single-protein molecular interactions on polymer-modified glass substrates for nanoarray chip application using dual-color TIRFM | - |
dc.title.alternative | Single-protein molecular interactions on polymer-modified glass substrates for nanoarray chip application using dual-color TIRFM | - |
dc.type | Article | - |
dc.citation.title | Bulletin of Korean Chemical Society | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 790 | - |
dc.citation.startPage | 783 | - |
dc.citation.volume | 28 | - |
dc.contributor.affiliatedAuthor | Hee Gu Lee | - |
dc.contributor.alternativeName | 김대광 | - |
dc.contributor.alternativeName | 이희구 | - |
dc.contributor.alternativeName | 정형일 | - |
dc.contributor.alternativeName | 강성호 | - |
dc.identifier.bibliographicCitation | Bulletin of Korean Chemical Society, vol. 28, no. 5, pp. 783-790 | - |
dc.identifier.doi | 10.5012/bkcs.2007.28.5.783 | - |
dc.subject.keyword | dual-color TIRFM | - |
dc.subject.keyword | nanoarray protein chip | - |
dc.subject.keyword | polymer-modified glass | - |
dc.subject.keyword | single-molecule detection | - |
dc.subject.local | Dual-color TIRFM | - |
dc.subject.local | dual-color TIRFM | - |
dc.subject.local | nanoarray protein chip | - |
dc.subject.local | Nanoarray protein chip | - |
dc.subject.local | polymer-modified glass | - |
dc.subject.local | single-molecule detection | - |
dc.subject.local | Single-molecule detection | - |
dc.description.journalClass | Y | - |
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