Rapid screen of human genes for relevance to cancer using fission yeast

Cited 12 time in scopus
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Title
Rapid screen of human genes for relevance to cancer using fission yeast
Author(s)
Kyung Sook Chung; Y J Jang; Nam-Soon Kim; S Y Park; Shin Jung Choi; J Y Kim; J H Ahn; H J Lee; J H Lim; S H Song; J H Ji; J H Oh; K B Song; Hyang Sook Yoo; Mi Sun Won
Bibliographic Citation
Journal of Biomolecular Screening, vol. 12, no. 4, pp. 568-577
Publication Year
2007
Abstract
A total of 437 human full-length cDNAs isolated by microarray analysis of liver and/or gastric cancer tissues were evaluated for their relevance to cancer using the fission yeast Schizosaccharomyces pombe. Overexpression of 161 human cDNAs in S. pombe caused growth inhibition and/or morphological changes, which can be considered as cancer-related phenotypes of S. pombe. Sixteen genes causing growth defects and morphological changes at the same time were chosen to validate their ostensible oncogenic properties. They were highly expressed in liver and/or gastric cancer cell lines. Also, when the mouse embryonic fibroblast cell type NIH3T3 was transfected with these genes, the proliferation rates of cells were increased by 32% to 120%. This study demonstrates that fission yeast can be used as an advantageous and powerful tool for the rapid screening of human genes relevant to cancer. Furthermore, the human genes screened can be tested further as diagnostic markers and potential therapeutic targets for liver and stomach cancers. They also can be studied further for the elucidation of mechanisms involved in carcinogenesis.
Keyword
cell-based assayfission yeastoncogenesisoverexpressionphenotype
ISSN
1087-0571
Publisher
Sage
DOI
http://dx.doi.org/10.1177/1087057107301007
Type
Article
Appears in Collections:
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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