Sequence-based screening for self-sufficient P450 monooxygenase from a metagenome library

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dc.contributor.authorB S Kim-
dc.contributor.authorS Y Kim-
dc.contributor.authorJ Park-
dc.contributor.authorW Park-
dc.contributor.authorK Y Hwang-
dc.contributor.authorY J Yoon-
dc.contributor.authorW K Oh-
dc.contributor.authorBo Yeon Kim-
dc.contributor.authorJong Seog Ahn-
dc.date.accessioned2017-04-19T09:07:37Z-
dc.date.available2017-04-19T09:07:37Z-
dc.date.issued2007-
dc.identifier.issn1364-5072-
dc.identifier.uri10.1111/j.1365-2672.2006.03169.xko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7961-
dc.description.abstractAims: Cytochrome P450 monooxygenases (CYPs) are useful catalysts for oxidation reactions. Self-sufficient CYPs harbour a reductive domain covalently connected to a P450 domain and are known for their robust catalytic activity with great potential as biocatalysts. In an effort to expand genetic sources of self-sufficient CYPs, we devised a sequence-based screening system to identify them in a soil metagenome. Methods and Results: We constructed a soil metagenome library and performed sequence-based screening for self-sufficient CYP genes. A new CYP gene, syk181, was identified from the metagenome library. Phylogenetic analysis revealed that SYK181 formed a distinct phylogenic line with 46% amino-acid-sequence identity to CYP102A1 which has been extensively studied as a fatty acid hydroxylase. The heterologously expressed SYK181 showed significant hydroxylase activity towards naphthalene and phenanthrene as well as towards fatty acids. Conclusions: Sequence-based screening of metagenome libraries is expected to be a useful approach for searching self-sufficient CYP genes. The translated product of syk181 shows self-sufficient hydroxylase activity towards fatty acids and aromatic compounds. Significance and Impact of the Study: SYK181 is the first self-sufficient CYP obtained directly from a metagenome library. The genetic and biochemical information on SYK181 are expected to be helpful for engineering self-sufficient CYPs with broader catalytic activities towards various substrates, which would be useful for bioconversion of natural products and biodegradation of organic chemicals.-
dc.publisherWiley-
dc.titleSequence-based screening for self-sufficient P450 monooxygenase from a metagenome library-
dc.title.alternativeSequence-based screening for self-sufficient P450 monooxygenase from a metagenome library-
dc.typeArticle-
dc.citation.titleJournal of Applied Microbiology-
dc.citation.number5-
dc.citation.endPage1400-
dc.citation.startPage1392-
dc.citation.volume102-
dc.contributor.affiliatedAuthorBo Yeon Kim-
dc.contributor.affiliatedAuthorJong Seog Ahn-
dc.contributor.alternativeName김범석-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName-
dc.contributor.alternativeName오원근-
dc.contributor.alternativeName김보연-
dc.contributor.alternativeName안종석-
dc.identifier.bibliographicCitationJournal of Applied Microbiology, vol. 102, no. 5, pp. 1392-1400-
dc.identifier.doi10.1111/j.1365-2672.2006.03169.x-
dc.subject.keywordfatty acid hydroxylase-
dc.subject.keywordmetagenome-
dc.subject.keywordself-sufficient cytochrome P450 monooxygenase-
dc.subject.keywordsequence-based screening-
dc.subject.localfatty acid hydroxylase-
dc.subject.localmetagenome-
dc.subject.localMetagenome-
dc.subject.localself-sufficient cytochrome P450 monooxygenase-
dc.subject.localsequence-based screening-
dc.description.journalClassY-
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Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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