DC Field | Value | Language |
---|---|---|
dc.contributor.author | B S Kim | - |
dc.contributor.author | S Y Kim | - |
dc.contributor.author | J Park | - |
dc.contributor.author | W Park | - |
dc.contributor.author | K Y Hwang | - |
dc.contributor.author | Y J Yoon | - |
dc.contributor.author | W K Oh | - |
dc.contributor.author | Bo Yeon Kim | - |
dc.contributor.author | Jong Seog Ahn | - |
dc.date.accessioned | 2017-04-19T09:07:37Z | - |
dc.date.available | 2017-04-19T09:07:37Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1364-5072 | - |
dc.identifier.uri | 10.1111/j.1365-2672.2006.03169.x | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7961 | - |
dc.description.abstract | Aims: Cytochrome P450 monooxygenases (CYPs) are useful catalysts for oxidation reactions. Self-sufficient CYPs harbour a reductive domain covalently connected to a P450 domain and are known for their robust catalytic activity with great potential as biocatalysts. In an effort to expand genetic sources of self-sufficient CYPs, we devised a sequence-based screening system to identify them in a soil metagenome. Methods and Results: We constructed a soil metagenome library and performed sequence-based screening for self-sufficient CYP genes. A new CYP gene, syk181, was identified from the metagenome library. Phylogenetic analysis revealed that SYK181 formed a distinct phylogenic line with 46% amino-acid-sequence identity to CYP102A1 which has been extensively studied as a fatty acid hydroxylase. The heterologously expressed SYK181 showed significant hydroxylase activity towards naphthalene and phenanthrene as well as towards fatty acids. Conclusions: Sequence-based screening of metagenome libraries is expected to be a useful approach for searching self-sufficient CYP genes. The translated product of syk181 shows self-sufficient hydroxylase activity towards fatty acids and aromatic compounds. Significance and Impact of the Study: SYK181 is the first self-sufficient CYP obtained directly from a metagenome library. The genetic and biochemical information on SYK181 are expected to be helpful for engineering self-sufficient CYPs with broader catalytic activities towards various substrates, which would be useful for bioconversion of natural products and biodegradation of organic chemicals. | - |
dc.publisher | Wiley | - |
dc.title | Sequence-based screening for self-sufficient P450 monooxygenase from a metagenome library | - |
dc.title.alternative | Sequence-based screening for self-sufficient P450 monooxygenase from a metagenome library | - |
dc.type | Article | - |
dc.citation.title | Journal of Applied Microbiology | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 1400 | - |
dc.citation.startPage | 1392 | - |
dc.citation.volume | 102 | - |
dc.contributor.affiliatedAuthor | Bo Yeon Kim | - |
dc.contributor.affiliatedAuthor | Jong Seog Ahn | - |
dc.contributor.alternativeName | 김범석 | - |
dc.contributor.alternativeName | 김 | - |
dc.contributor.alternativeName | 박 | - |
dc.contributor.alternativeName | 박 | - |
dc.contributor.alternativeName | 황 | - |
dc.contributor.alternativeName | 윤 | - |
dc.contributor.alternativeName | 오원근 | - |
dc.contributor.alternativeName | 김보연 | - |
dc.contributor.alternativeName | 안종석 | - |
dc.identifier.bibliographicCitation | Journal of Applied Microbiology, vol. 102, no. 5, pp. 1392-1400 | - |
dc.identifier.doi | 10.1111/j.1365-2672.2006.03169.x | - |
dc.subject.keyword | fatty acid hydroxylase | - |
dc.subject.keyword | metagenome | - |
dc.subject.keyword | self-sufficient cytochrome P450 monooxygenase | - |
dc.subject.keyword | sequence-based screening | - |
dc.subject.local | fatty acid hydroxylase | - |
dc.subject.local | metagenome | - |
dc.subject.local | Metagenome | - |
dc.subject.local | self-sufficient cytochrome P450 monooxygenase | - |
dc.subject.local | sequence-based screening | - |
dc.description.journalClass | Y | - |
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