Characterization of DNA methylation change in stem cell marker genes during differentiation of human embryonic stem cells

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Title
Characterization of DNA methylation change in stem cell marker genes during differentiation of human embryonic stem cells
Author(s)
Seungeun Yeo; Sangkyun Jeong; Janghwan Kim; J S Han; Y M Han; Yong-Kook Kang
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 359, no. 3, pp. 536-542
Publication Year
2007
Abstract
Pluripotent human embryonic stem cells (hESCs) have the distinguishing feature of innate capacity to allow indefinite self-renewal. This attribute continues until specific constraints or restrictions, such as DNA methylation, are imposed on the genome, usually accompanied by differentiation. With the aim of utilizing DNA methylation as a sign of early differentiation, we probed the genomic regions of hESCs, particularly focusing on stem cell marker (SCM) genes to identify regulatory sequences that display differentiation-sensitive alterations in DNA methylation. We show that the promoter regions of OCT4 and NANOG, but not SOX2, REX1 and FOXD3, undergo significant methylation during hESCs differentiation in which SCM genes are substantially repressed. Thus, following exposure to differentiation stimuli, OCT4 and NANOG gene loci are modified relatively rapidly by DNA methylation. Accordingly, we propose that the DNA methylation states of OCT4 and NANOG sequences may be utilized as barometers to determine the extent of hESC differentiation.
Keyword
DNA methylationHuman embryonic stem cellNanogOct4
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2007.05.120
Type
Article
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Aging Convergence Research Center > 1. Journal Articles
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