Real-time monitoring of cell-free protein synthesis on a surface plasmon resonance chip

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Title
Real-time monitoring of cell-free protein synthesis on a surface plasmon resonance chip
Author(s)
K H Lee; Hyou-Arm Joung; J H Ahn; K O Kim; I S Oh; Yong Beom Shin; Min-Gon Kim; D M Kim
Bibliographic Citation
Analytical Biochemistry, vol. 366, no. 2, pp. 170-174
Publication Year
2007
Abstract
Taking advantage of the "open" nature of cell-free protein synthesis, this study investigated the direct analysis of protein expression using a surface plasmon resonance sensor. During the on-chip incubation of the reaction mixture for cell-free protein synthesis, the expressed protein molecules were immobilized onto the surface of the chip, giving rise to a sensorgram signal, which enabled on-line monitoring of protein expression. In addition, we found that the expression of the aggregation-prone proteins could be effectively monitored. The ability to monitor these proteins was most likely through the instant isolation of the expressed protein molecules onto the solid surface of the chip.
Keyword
cell-free protein synthesisreal-time monitoringSPR
ISSN
0003-2697
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.ab.2007.04.044
Type
Article
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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