Lignans and coumarins from the roots of Anthriscus sylvestris and their increase of Caspase-3 activity in HL-60 cells

Cited 38 time in scopus
Metadata Downloads
Title
Lignans and coumarins from the roots of Anthriscus sylvestris and their increase of Caspase-3 activity in HL-60 cells
Author(s)
G S Jeong; Ok-Kyoung Kwon; Bo-Young Park; Sei Ryang OhKyung Seop Ahn; M J Chang; W K Oh; J C Kim; B S Min; Y C Kim; Hyeong Kyu Lee
Bibliographic Citation
Biological & Pharmaceutical Bulletin, vol. 30, no. 7, pp. 1340-1343
Publication Year
2007
Abstract
A new lignan, sylvestrin (1), was isolated from the MeOH-soluble fraction of the roots of Anthriscus sylvestris HOFFM. (Umbelliferae), along with six lignans (2-7), three coumarins (8-10), and a polyacetylene (11). The structure of sylvestrin was determined to be 2-(3′,4′,5′- trimethoxybenzylidene)-3-(3″,4″-methylendioxybenzyl) -γ-butyrolactone (1) by spectroscopic means, including 2D-NMR. The eleven compounds were assessed for their abilities to activate a caspase-3 in human promyeloid leukemic HL-60 cells. The intracellular caspase-3 activity of (-)-deoxypodophyllotoxin (3), angeloyl podophyllotoxin (5), deoxypicropodophyllin (6), picropodophyllotoxin (7), and falcarindiol (11) increased approximately 4.6, 3.6, 3.7, 3.9, and 3.9-fold, at 0.001, 1, 1, 1, and 20 μM, respectively, over that of the untreated control. In addition, compounds 3, 5, 6, and 7 showed apoptosis-inducing activities that were measured by DNA fragmentation in HL-60 cells.
Keyword
anthriscus sylvestrisapoptosiscaspase-3DNA fragmentationsylvestrinumbelliferae
ISSN
0918-6158
Publisher
Pharmaceutical Soc Japan
DOI
http://dx.doi.org/10.1248/bpb.30.1340
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Bio-Resource Central Bank > 1. Journal Articles
Ochang Branch Institute > 1. Journal Articles
Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.