Relationship between genome similarity and DNA-DNA hybridization among closely related bacteria

Cited 0 time in scopus
Metadata Downloads
Title
Relationship between genome similarity and DNA-DNA hybridization among closely related bacteria
Author(s)
C H Kang; Young-Do Nam; W H Chung; Z X Quan; Yong Ha Park; S J Park; R Desmone; X F Wan; S K Rhee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 17, no. 6, pp. 945-951
Publication Year
2007
Abstract
DNA-DNA hybridization has been established as an important technology in bacterial species taxonomy and phylogenetic analysis. In this study, we analyzed how the efficiency with which the genomic DNA from one species hybridizes to the genomic DNA of another species (DNA-DNA hybridization) in microarray analysis relates to the similarity between two genomes. We found that the predicted DNA-DNA hybridization based on genome sequence similarity correlated well with the experimentally determined microarray hybridization. Between closely related strains, significant numbers of highly divergent genes (<55% identity) and/or the accumulation of mismatches between conserved genes lowered the DNA-DNA hybridization signal, and this reduced the hybridization signals to below 70% for even bacterial strains with over 97% 16S rRNA gene identity. In addition, our results also suggest that a DNA-DNA hybridization signal intensity of over 40% indicates that two genomes at least shared 30% conserved genes (>60% gene idemity). This study may expand our knowledge of DNA-DNA hybridization based on genomic sequence similarity comparison and further provide insights for bacterial phylogeny analyses.
Keyword
DNA microarraygenome similaritypredicted DNA-DNA hybridization signal
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.