Cited 0 time in
- Title
- Relationship between genome similarity and DNA-DNA hybridization among closely related bacteria
- Author(s)
- C H Kang; Young-Do Nam; W H Chung; Z X Quan; Yong Ha Park; S J Park; R Desmone; X F Wan; S K Rhee
- Bibliographic Citation
- Journal of Microbiology and Biotechnology, vol. 17, no. 6, pp. 945-951
- Publication Year
- 2007
- Abstract
- DNA-DNA hybridization has been established as an important technology in bacterial species taxonomy and phylogenetic analysis. In this study, we analyzed how the efficiency with which the genomic DNA from one species hybridizes to the genomic DNA of another species (DNA-DNA hybridization) in microarray analysis relates to the similarity between two genomes. We found that the predicted DNA-DNA hybridization based on genome sequence similarity correlated well with the experimentally determined microarray hybridization. Between closely related strains, significant numbers of highly divergent genes (<55% identity) and/or the accumulation of mismatches between conserved genes lowered the DNA-DNA hybridization signal, and this reduced the hybridization signals to below 70% for even bacterial strains with over 97% 16S rRNA gene identity. In addition, our results also suggest that a DNA-DNA hybridization signal intensity of over 40% indicates that two genomes at least shared 30% conserved genes (>60% gene idemity). This study may expand our knowledge of DNA-DNA hybridization based on genomic sequence similarity comparison and further provide insights for bacterial phylogeny analyses.
- Keyword
- DNA microarraygenome similaritypredicted DNA-DNA hybridization signal
- ISSN
- 1017-7825
- Publisher
- Korea Soc-Assoc-Inst
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.