Surface plasmon resonance biosensor chips

Abstract

In this study, we describe biosensors predicated on surface plasmon resonance (SPR), a phenomenon that has recently become a focus of interest in biomedical science research. SPR has been shown to occur on two-dimensional metal surfaces, which support the SPR-active substrate on a glass prism. The SPR biosensor, which exploits the SPR effect, is a label-free and surface-sensitive spectroscopic system that utilizes measured changes in the local refraction index upon adsorption. Based on the SPR phenomenon, other technologies have been developed, including SPR-MS, SPR microscopy, localized SPR, and SPR imaging. The development of an SPR imaging system has already begun to overcome one of the difficulties inherent to conventional SPR, namely its high-throughput limitation. In this review, the detection principles and properties of SPR biosensors are briefly elucidated, and the possible future biomedical applications of SPR biosensors, including high-throughput screening in drug discovery, analysis of biomolecular interactions, and proteomics research are also discussed. This discussion is particularly salient now that SPR imaging provides another useful tool for the performance of high-throughput assays.