Characterization of levan hydrolysis activity of levansucrase from Zymomonas mobilis ATCC 10988 and Rahnella aquatilis ATCC 33071

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Title
Characterization of levan hydrolysis activity of levansucrase from Zymomonas mobilis ATCC 10988 and Rahnella aquatilis ATCC 33071
Author(s)
K H Jang; S A Kang; Chul Ho Kim; J C Lee; M H Kim; E Son; Sang Ki Rhee
Bibliographic Citation
Food Science and Biotechnology, vol. 16, no. 3, pp. 482-484
Publication Year
2007
Abstract
To investigate production and hydrolysis of levan, the levansucrase enzymes from Zymomonas mobilis ATCC 10988 and Rahnella aquatilis ATCC 33071 were used. The optimum temperature of R. aquatilis levansucrase for levan formation was 37°C, whereas that of Z. mobilis was 4°C, under the experimental conditions. Both levansucrases also catalyzed the reverse levan hydrolysis reaction. Levan hydrolysis reactions from both levansucrases were temperature dependent; high temperature (20°C) was more favorable than low temperature (4°C) by 4 times. Fructose was the only product from hydrolysis reaction by both levansucrases, showing that both levansucrases mediated the hydrolysis reaction of exo-enzyme acting. In both enzymes, initial levan hydrolysis activity was almost accounted to 1% of initial levan formation activity. The results allow the estimation of the fructose release rate in enzyme processing conditions.
Keyword
hydrolysis of levanlevanlevansucrasetransfructosylationrahnella aquatiliszymomonas mobilis
ISSN
1226-7708
Publisher
South Korea
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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