Screening of a specific monoclonal antibody against and detection of Listeria monocytogenes whole cells using a surface plasmon resonance biosensor

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Title
Screening of a specific monoclonal antibody against and detection of Listeria monocytogenes whole cells using a surface plasmon resonance biosensor
Author(s)
H A Joung; W B Shim; D H Chung; Jun Hyoung Ahn; Bong Hyun Chung; H S Choi; S D Ha; K S Kim; K H Lee; C H Kim; K Y Kim; Min-Gon Kim
Bibliographic Citation
Biotechnology Bioprocess Engineering, vol. 12, no. 2, pp. 80-85
Publication Year
2007
Abstract
In this study, a specific monoclonal antibody against Listeria monocytogenes was screened using an SPR biosensor. Monoclonal antibodies were bound to protein L, after which the L. monocytogenes cells were subjected to an affinity assay. Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surface via an arnine coupling method, and utilized repeatedly by regeneration. The monoclonal antibody, 'A18', was selected and employed for the high-sensitivity detection of L. monocytogenes. Under optimized conditions, 103 cells/mL or 50 cells were detected by the SPR biosensor.
Keyword
antibodylisteria monocytogenesprotein Lsurface plasmon resonance (SPR)
ISSN
I000-0116
Publisher
South Korea
DOI
http://dx.doi.org/10.1007/BF03028630
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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