The role of water channel proteins and nitric oxide signaling in rice seed germination

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Title
The role of water channel proteins and nitric oxide signaling in rice seed germination
Author(s)
H Y Liu; X Yu; D Y Cui; M H Sun; W N Sun; Z C Tang; Sang Soo Kwak; W A Su
Bibliographic Citation
Cell Research, vol. 17, no. 7, pp. 638-649
Publication Year
2007
Abstract
Previous studies have demonstrated the possible role of several aquaporins in seed germination. But systematic investigation of the role of aquaporin family members in this process is lacking. Here, the developmental regulation of plasma membrane intrinsic protein (PIP) expression throughout germination and post-germination processes in rice embryos was analyzed. The expression patterns of the PIPs suggest these aquaporins play different roles in seed germination and seedling growth. Partial silencing of the water channel genes, OsPIP1;1 and OsPIP1;3, reduced seed germination while over-expression of OsPIP1;3 promoted seed germination under water-stress conditions. Moreover, spatial expression analysis indicates that OsPIP1;3 is expressed predominantly in embryo during seed germination. Our data also revealed that the nitric oxide (NO) donors, sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO), promoted seed germination; furthermore, the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide, inhibited germination and reduced the stimulative effects of SNP and GSNO on rice germination. Exogenous NO stimulated the transcription of OsPIP1;1, OsPIP1;2, OsPIP1;3 and OsPIP2;8 in germinating seeds. These results suggest that water channels play an important role in seed germination, acting, at least partly, in response to the NO signaling pathway.
Keyword
germinationnitric oxideoryza sativaover-expressionsilencingwater channel
ISSN
1001-0602
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/cr.2007.34
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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