Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans

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dc.contributor.authorY Song-
dc.contributor.authorM H Choi-
dc.contributor.authorJeong Nam Park-
dc.contributor.authorMoo Woong Kim-
dc.contributor.authorEun Jung Kim-
dc.contributor.authorHyun Ah Kang-
dc.contributor.authorJ Y Kim-
dc.date.accessioned2017-04-19T09:07:45Z-
dc.date.available2017-04-19T09:07:45Z-
dc.date.issued2007-
dc.identifier.issn0099-2240-
dc.identifier.uri10.1128/AEM.02058-06ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/8004-
dc.description.abstractIn an attempt to engineer a Yarrowia lipolytica strain to produce glycoproteins lacking the outer-chain mannose residues of N-linked oligosaccharides, we investigated the functions of the OCH1 gene encoding a putative α-1,6-mannosyltransferase in Y. lipolytica. The complementation of the Saccharomyces cerevisiae och1 mutation by the expression of YlOCH1 and the lack of in vitro α-1,6-mannosyltransferase activity in the Yloch1 null mutant indicated that YlOCH1 is a functional ortholog of S. cerevisiae OCH1. The oligosaccharides assembled on two secretory glycoproteins, the Trichoderma reesei endoglucanase I and the endogenous Y. lipolytica lipase, from the Yloch1 null mutant contained a single predominant species, the core oligosaccharide Man8GlcNAc2, whereas those from the wild-type strain consisted of oligosaccharides with heterogeneous sizes, Man 8GlcNAc2 to Man12GlcNAc2. Digestion with α-1,2- and α-1,6-mannosidase of the oligosaccharides from the wild-type and Yloch1 mutant strains strongly supported the possibility that the Yloch1 mutant strain has a defect in adding the first α-1,6-linked mannose to the core oligosaccharide. Taken together, these results indicate that YlOCH1 plays a key role in the outer-chain mannosylation of N-linked oligosaccharides in Y. lipolytica. Therefore, the Yloch1 mutant strain can be used as a host to produce glycoproteins lacking the outer-chain mannoses and further developed for the production of therapeutic glycoproteins containing human-compatible oligosaccharides.-
dc.publisherAmer Soc Microb-
dc.titleEngineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans-
dc.title.alternativeEngineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans-
dc.typeArticle-
dc.citation.titleApplied and Environmental Microbiology-
dc.citation.number14-
dc.citation.endPage4454-
dc.citation.startPage4446-
dc.citation.volume73-
dc.contributor.affiliatedAuthorJeong Nam Park-
dc.contributor.affiliatedAuthorMoo Woong Kim-
dc.contributor.affiliatedAuthorEun Jung Kim-
dc.contributor.affiliatedAuthorHyun Ah Kang-
dc.contributor.alternativeName송윤경-
dc.contributor.alternativeName최민희-
dc.contributor.alternativeName박정남-
dc.contributor.alternativeName김무웅-
dc.contributor.alternativeName김은중-
dc.contributor.alternativeName강현아-
dc.contributor.alternativeName김정윤-
dc.identifier.bibliographicCitationApplied and Environmental Microbiology, vol. 73, no. 14, pp. 4446-4454-
dc.identifier.doi10.1128/AEM.02058-06-
dc.description.journalClassY-
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