DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seung-Wook Chi | - |
dc.contributor.author | C Y Maeng | - |
dc.contributor.author | Seung Jun Kim | - |
dc.contributor.author | M S Oh | - |
dc.contributor.author | C J Ryu | - |
dc.contributor.author | Sang Jick Kim | - |
dc.contributor.author | Kyou Hoon Han | - |
dc.contributor.author | Hyo Jeong Hong | - |
dc.contributor.author | Seong Eon Ryu | - |
dc.date.accessioned | 2017-04-19T09:07:45Z | - |
dc.date.available | 2017-04-19T09:07:45Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | 10.1073/pnas.0701279104 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8006 | - |
dc.description.abstract | The humanized monoclonal antibody HzKR127 recognizes the preS1 domain of the human hepatitis B virus surface proteins with a broadly neutralizing activity in vivo. We present the crystal structures of HzKR127 Fab and its complex with a major epitope peptide. In the complex structure, the bound peptide forms a type IV β-turn followed by 310 helical turn, the looped-out conformation of which provides a structural basis for broad neutralization. Upon peptide binding, the antibody undergoes a dramatic complementarity determining region H3 lid opening. To understand the structural implication of the virus neutralization, we carried out comprehensive alanine-scanning mutagenesis of all complementarity determining region residues in HzKR127 Fab. The functional mapping of the antigen-combining site demonstrates the specific roles of major binding determinants in antigen binding, contributing to the rational design for maximal humanization and affinity maturation of the antibody. | - |
dc.publisher | Natl Acad Sciences | - |
dc.title | Broadly neutralizing anti-hepatitis B virus antibody reveals a complementarity determining region H3 lid-opening mechanism | - |
dc.title.alternative | Broadly neutralizing anti-hepatitis B virus antibody reveals a complementarity determining region H3 lid-opening mechanism | - |
dc.type | Article | - |
dc.citation.title | Proceedings of National Academy of Sciences of United States of America | - |
dc.citation.number | 22 | - |
dc.citation.endPage | 9235 | - |
dc.citation.startPage | 9230 | - |
dc.citation.volume | 104 | - |
dc.contributor.affiliatedAuthor | Seung-Wook Chi | - |
dc.contributor.affiliatedAuthor | Seung Jun Kim | - |
dc.contributor.affiliatedAuthor | Sang Jick Kim | - |
dc.contributor.affiliatedAuthor | Kyou Hoon Han | - |
dc.contributor.affiliatedAuthor | Hyo Jeong Hong | - |
dc.contributor.affiliatedAuthor | Seong Eon Ryu | - |
dc.contributor.alternativeName | 지승욱 | - |
dc.contributor.alternativeName | 맹철영 | - |
dc.contributor.alternativeName | 김승준 | - |
dc.contributor.alternativeName | 오미숙 | - |
dc.contributor.alternativeName | 류춘제 | - |
dc.contributor.alternativeName | 김상직 | - |
dc.contributor.alternativeName | 한규훈 | - |
dc.contributor.alternativeName | 홍효정 | - |
dc.contributor.alternativeName | 류성언 | - |
dc.identifier.bibliographicCitation | Proceedings of National Academy of Sciences of United States of America, vol. 104, no. 22, pp. 9230-9235 | - |
dc.identifier.doi | 10.1073/pnas.0701279104 | - |
dc.subject.keyword | Alanine-scanning mutagenesis | - |
dc.subject.keyword | Crystal structure | - |
dc.subject.keyword | Humanized antibody | - |
dc.subject.keyword | Virus neutralization | - |
dc.subject.local | Alanine-scanning mutagenesis | - |
dc.subject.local | crystal structure | - |
dc.subject.local | Crystal structure | - |
dc.subject.local | Humanized antibody | - |
dc.subject.local | humanized antibodies | - |
dc.subject.local | humanized antibody | - |
dc.subject.local | Virus neutralization | - |
dc.description.journalClass | Y | - |
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