The analysis and application of a recombinant monooxygenase library as a biocatalyst for the Baeyer-Villiger reaction = Recombinant monooxygenase library의 분석 및 응용

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Title
The analysis and application of a recombinant monooxygenase library as a biocatalyst for the Baeyer-Villiger reaction = Recombinant monooxygenase library의 분석 및 응용
Author(s)
J Park; D Kim; SuJin Kim; Jinhee Kim; K Bae; C Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 17, no. 7, pp. 1083-1089
Publication Year
2007
Abstract
Because of their selectivity and catalytic efficiency, BVMOs are highly valuable biocatalysts for the chemoenzymatic synthesis of a broad range of useful compounds. In this study, we investigated the microbial Baeyer-Villiger oxidation and sulfoxidation of thioanisole and bicyclo[3.2.0]hept-2-en-6-one using whole Escherichia coli cells that recombined with each of the Baeyer-Villiger monooxygenases originated from Pseudomonas aeruginosa PAO1 and two from Streptomyces coelicolor A3(2). The three BVMOs were identified in the microbial genome database by a recently described protein sequence motif; e.g., BVMO motif (FXGXXXHXXXW). The reaction products were identified as (R)-/ (S)-sulfoxide and 2-oxabicyclo/3-oxabicyclo[3.3.0]oct-6-en-2-one by GC-MS analysis. Consequently, this study demonstrated that the three enzymes can indeed catalyze the Baeyer-Villiger reaction as a biocatalyst, and effective annotation tools can be efficiently exploited as a source of novel BVMOs.
Keyword
baeyer-Villiger oxidationbiocatalysisGC-MSmonooxygenasesulfide oxidation
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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