In vitro biosynthesis of strictosidine using Lonicera japonica leaf extracts and recombinant yeast

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Title
In vitro biosynthesis of strictosidine using Lonicera japonica leaf extracts and recombinant yeast
Author(s)
K H Nam; H J Chung; E J Jeon; M K Park; Y H Yim; Jang Ryol Liu; J H Park
Bibliographic Citation
Journal of Plant Biology, vol. 50, no. 3, pp. 315-320
Publication Year
2007
Abstract
Strictosidine is a key intermediate in the biosynthesis of the terpenoid indole alkaloid (TIA) pathway. It results from a condensation reaction, catalyzed by strictosidine synthase (STR), between tryptamine and secologanin. We have now developed a useful method, based on enzyme-assisted synthesis, to produce strictosidine. Our procedure utilizes leaf extracts from Japanese honeysuckle Lonicera japonica Thunb. as a secologanin source. In these experiments, an enzyme extract was prepared from transgenic yeast Saccharomyces cerevisiae that expresses the Catharanthus roseus STR (CrSTR) coding region. Strictosidine was then isolated with a 38% yield based on the initial amount of tryptamine in the enzymatic reaction.
Keyword
Catharanthus roseusHoneysuckle Lonicera japonicaSecologaninStrictosidineStrictosidine synthase
ISSN
I000-0094
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF03030660
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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