Optimal culture conditions on the tyrosinase inhibitor production by actinomycetes F-97 = 방선균 F-97에 의한 Tyrosinase 저해제 생성 최적 배양 조건

Abstract

A Actinomycetes F-97 producing tyrosinase inhibitor was isolated from soil samples. The optimum culture condition for tyrosinase inhibitor production was investigated and the results were as follows. The best carbon source for tyrosinase inhibitor production was shown as soluble starch, the optimum concentration was 3.0%. The best nitrogen source for tyrosinase inhibitor production was shown as peptone, the optimum concentration was 0.36%. As effect of metal ions on the production of tyrosinase inhibitor, K2HPO4 was shown the best and the optimum concentration was 0.1 mM. The optimum pH and temperature was shown 7.0 and 30℃, respectively. And the highest tyrosinase inhibitor production was observed at 70 hr cultivation under optimum conditions in jar fermentor scale.