DC Field | Value | Language |
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dc.contributor.author | Sung Ran Min | - |
dc.contributor.author | Jang Ryol Liu | - |
dc.contributor.author | Suk Weon Kim | - |
dc.date.accessioned | 2017-04-19T09:07:50Z | - |
dc.date.available | 2017-04-19T09:07:50Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1863-5466 | - |
dc.identifier.uri | 10.1007/s11816-006-0005-0 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/8041 | - |
dc.description.abstract | Culture conditions for high frequency plant regeneration via somatic embryogenesis from cell suspension cultures of Ranunculus kazusensis are described. Zygotic embryos formed white nodular structures and pale-yellow calluses at a frequency of 84.9% when cultured on half-strength Schenk and Hildebrandt (SH) medium supplemented with 0.1 mg l.1 2,4-dichlorophenoxyacetic acid (2,4-D). However, the frequency of white nodular structure and off-white callus formation decreased with an increasing concentration of 2,4-D up to 10 mg l.1, when the frequency reached 25%. Cell suspension cultures were established from zygotic embryo-derived pale-yellow calluses using half-strength SH medium supplemented with 0.1 mg l.1 of 2,4-D. Upon plating onto halfstrength SH basal medium, over 90% of cell aggregates gave rise to numerous somatic embryos and developed into plantlets. Regenerated plantlets were successfully transplanted to potting soil and grown to maturity at a survival rate of over 90% in a growth chamber. The plant regeneration system established in this study can be applied to mass propagation and conservation of this species. | - |
dc.publisher | Springer | - |
dc.title | Plant regeneration from zygotic embryo-derived embryogenic cell suspension cultures of Ranunculus kazusensis | - |
dc.title.alternative | Plant regeneration from zygotic embryo-derived embryogenic cell suspension cultures of Ranunculus kazusensis | - |
dc.type | Article | - |
dc.citation.title | Plant Biotechnology Reports | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 60 | - |
dc.citation.startPage | 57 | - |
dc.citation.volume | 1 | - |
dc.contributor.affiliatedAuthor | Sung Ran Min | - |
dc.contributor.affiliatedAuthor | Jang Ryol Liu | - |
dc.contributor.affiliatedAuthor | Suk Weon Kim | - |
dc.contributor.alternativeName | 민성란 | - |
dc.contributor.alternativeName | 유장렬 | - |
dc.contributor.alternativeName | 김석원 | - |
dc.identifier.bibliographicCitation | Plant Biotechnology Reports, vol. 1, no. 1, pp. 57-60 | - |
dc.identifier.doi | 10.1007/s11816-006-0005-0 | - |
dc.subject.keyword | ranunculus kazusensis | - |
dc.subject.keyword | plant regeneration | - |
dc.subject.keyword | somatic embryogenesis | - |
dc.subject.keyword | suspension culture | - |
dc.subject.local | ranunculus kazusensis | - |
dc.subject.local | plant regeneration | - |
dc.subject.local | Plant regeneration | - |
dc.subject.local | somatic embryogenisis | - |
dc.subject.local | somatic embryogenesis | - |
dc.subject.local | Somatic embryogenesis | - |
dc.subject.local | Suspension cultures | - |
dc.subject.local | Suspension culture | - |
dc.subject.local | suspension culture | - |
dc.description.journalClass | Y | - |
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