Discrimination of higher plant calluses based on embryogenic capacity and taxonomic classification by pyrolysis mass spectrometry

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Title
Discrimination of higher plant calluses based on embryogenic capacity and taxonomic classification by pyrolysis mass spectrometry
Author(s)
Suk Weon Kim; S H Ban; O J Yoo; Jang Ryol Liu
Bibliographic Citation
Plant Biotechnology Reports, vol. 1, no. 1, pp. 61-65
Publication Year
2007
Abstract
Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum, and has been widely applied to the discrimination of closely related microbial strains. Minimally prepared samples of embryogenic and non-embryogenic calluses derived from various higher plants (sweet potato, morning glory, Korean ginseng, Siberian ginseng, and balloon flower) were subjected to PyMS for spectral fingerprinting. A dendrogram based on the unweighted pair group method, with arithmetic mean of pyrolysis mass spectra, divided the calluses into Siberian ginseng embryogenic callus and the others, which were subsequently divided into embryogenic and nonembryogenic callus groups, regardless of plant species from which the calluses were derived. In the nonembryogenic callus group, the dendrogram was in agreement with the known taxonomy of the plants. These results indicate that PyMS analysis could be applied for discriminating plant calluses based on embryogenic capacity and taxonomic classification.
Keyword
canonical variate analysismultivariate analysisprincipal component analysis
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-007-0006-7
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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