High frequency somatic embryogenesis and plant regeneration in root explant cultures of carnation

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Title
High frequency somatic embryogenesis and plant regeneration in root explant cultures of carnation
Author(s)
J Seo; Suk Weon KimSung Ran Min; Jang Ryol Liu
Bibliographic Citation
Plant Biotechnology Reports, vol. 1, no. 2, pp. 67-70
Publication Year
2007
Abstract
Root explants excised from carnation plants maintained in vitro formed off-white, friable calluses after three weeks of culture on Murashige and Skoog (MS) medium supplemented with 1 mg l.1 thidiazuron (TDZ) and 1 mg l.1 a-naphthalaneacetic acid (NAA). These calluses were subsequently transferred to MS basal medium where, after an additional four weeks of culture, approximately 50% of the calluses formed somatic embryos. However, calluses formed on root explants that had been cultured on MS medium supplemented with 2,4-dichlorophenoxyacetic acid did not produce somatic embryos upon transfer to MS basal medium. Somatic embryos developed into plantlets and subsequently were grown to maturity. These results indicate that root explants have a high competence for somatic embryogenesis in carnation.
Keyword
dianthus caryophyllussomatic embryo
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-007-0010-y
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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